S0751

Sigma-Aldrich

Sodium phosphate monobasic

ReagentPlus®, ≥99.0%

Sinónimos:
Monosodium phosphate, Sodium dihydrogen phosphate, Monosodium dihydrogen orthophosphate
Fórmula lineal:
NaH2PO4
Número de CAS:
Peso molecular:
119.98
EC Number:
MDL number:
eCl@ss:
38070208
PubChem Substance ID:

Quality Level

product line

ReagentPlus®

assay

≥99.0%

form

powder

application(s)

DNA extraction: suitable
detection: suitable
electrophoresis: suitable
immunocytochemistry: suitable

pH

4.0-4.5 (25 °C, 50 g/L in water)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

water: 100 mg/mL, clear, colorless

SMILES string

[Na+].OP(O)([O-])=O

InChI

1S/Na.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1

InChI key

AJPJDKMHJJGVTQ-UHFFFAOYSA-M

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General description

Sodium phosphate monobasic acts as an acidulant in laxatives and effervescent powders. It is used in beverages, cheese and soft drink formulations.

Application

Sodium phosphate monobasic has been used:
  • as a reagent for virus transfection and fiber implantation in mouse
  • in phosphate buffer for immunocytochemistry and to detect individual catalase activity by PAGE (polyacrylamide gel electrophoresis)
  • as a buffer for cycling assay
  • in automated DNA extraction method
  • as a chemical in artificial cerebro-spinal fluid (aCSF) solution for the preparation of brainstem-spinal cord from rats

Packaging

3 kg in poly drum
1 kg in poly bottle
100, 500 g in poly bottle

Legal Information

ReagentPlus is a registered trademark of Sigma-Aldrich Co. LLC
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificado de Análisis

Certificado de origen

Two Different Methods of Quantification of Oxidized Nicotinamide Adenine Dinucleotide (NAD+) and Reduced Nicotinamide Adenine Dinucleotide (NADH) Intracellular Levels: Enzymatic Coupled Cycling Assay and Ultra-performance Liquid Chromatography (UPLC)-Mass Spectrometry
Kanamori KS, et al.
Bio-protocol, 8(14) (2018)
In vitro Brainstem-spinal Cord Preparation from Newborn Rat
Le Gal JP, et al.
The Journal of Neuroscience (2016)
Mild blast injury produces acute changes in basal intracellular calcium levels and activity patterns in mouse hippocampal neurons
Hansen KR, et al.
Journal of Neurotrauma, 35(13), 1523-1536 (2018)
Automated and manual methods of DNA extraction for Aspergillus fumigatus and Rhizopus oryzae analyzed by quantitative real-time PCR
Francesconi A, et al.
Journal of Clinical Microbiology, 46(6), 1978-1984 (2008)
Detection of Catalase Activity by Polyacrylamide Gel Electrophoresis (PAGE) in Cell Extracts from Pseudomonas aeruginosa
Pezzoni M, et al.
Microbiology (2016)
Protocolos
To standardize a procedure for the enzymatic assay of Urease, from Jack Beans
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Protocol for the enzymatic Trypsinogen Assay
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To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.
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Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. We offer a wide variety of convenient kits and reagents for rapid and reliable enzymatic food analysis.
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