T9284

Sigma-Aldrich

Triton X-100

BioXtra

Sinónimos:
t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Fórmula lineal:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Número de CAS:
Beilstein/REAXYS Number:
2315025
MDL number:
PubChem Substance ID:
NACRES:
NA.21

Quality Level

description

non-ionic

product line

BioXtra

form

liquid

mol wt

micellar avg mol wt 80,000
average mol wt 625

aggregation number

100-155

application(s)

permeability assay: suitable

impurities

≤0.05% Phosphorus (P)

ign. residue

≤0.4%

CMC

0.2-0.9 mM (20-25°C)

transition temp

cloud point 65 °C
pour point ~7 °C

solubility

water: soluble

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.1%
Pb: ≤0.001%
Zn: ≤0.0005%

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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Categorías relacionadas

General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton X-100 has been used:
  • Along with Span® 80 and Tween® 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique
  • As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling
  • In the blocking solution during immunocytochemistry procedure
  • During deglycosylation procedure
  • For membrane solubilization
  • For cell permeabilization
  • In decellularization of porcine heart valve tissue

Packaging

1 L in poly bottle
100, 500 mL in poly bottle

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Span is a registered trademark of Croda International PLC
TWEEN is a registered trademark of Croda International PLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

signalword

Danger

hazcat

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 3

Flash Point F

483.8 °F

Flash Point C

251 °C

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificado de Análisis

Certificado de origen

Detection of fetal DNA in maternal plasma by microarray coupled with emulsions PCR.
Ge Q, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 369(1), 82-88 (2006)
Structural, metabolic and developmental evaluation of ovulated rabbit oocytes before and after cryopreservation by vitrification and slow freezing.
Theriogenology, 74(5), 847-855 (2010)
Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations.
Dressman D, et al.
Proceedings of the National Academy of Sciences of the USA, 100(15), 8817-8822 (2003)
Proinflammatory chemokine induction in keratocytes and inflammatory cell infiltration into the cornea.
Hong JW et al.
Investigative Ophthalmology & Visual Science, 42, 2795-2795 (2001)
Angiotensin II AT1 receptor blockade decreases vasopressin-induced water reabsorption and AQP2 levels in NaCl-restricted rats.
Kwon TH et al.
American Journal of Physiology: Renal Physiology, 288, F673-F673 (2005)
Artículos
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Protocolos
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