05066

Agarose

High EEO, for molecular biology

• Synonym(s): 3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE
• CAS Number: 9012-36-6
• Número de EC: 232-731-8
• Número MDL: MFCD00081294
• ID de la sustancia en PubChem: 24845663
• NACRES: NA.25

Properties

• grado: for molecular biology
• Nivel de calidad: 100
• formulario: powder
• impurezas: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• residuo de ign.: ≤1%
• pérdida: ≤10% loss on drying
• ELECTROENDÓSMOSIS (EEO): 0.23-0.27
• temperatura de transición: gel point 34-37 °C (1.5% solution)
• fuerza de gel: ≥1500 g/cm²
• trazas de anión: chloride (Cl^-): ≤0.3%; sulfate (SO₄^2-): ≤0.6%
• trazas de catión: Ca: ≤0.05%; Cd: ≤0.001%; Co: ≤0.001%; Cr: ≤0.001%; Cu: ≤0.001%; Fe: ≤0.001%; K: ≤0.05%; Mg: ≤0.001%; Mn: ≤0.001%; Na: ≤0.5%; Ni: ≤0.001%; Pb: ≤0.001%; Zn: ≤0.001%
• InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
• InChI key: MJQHZNBUODTQTK-WKGBVCLCSA-N

Description

Aplicación

Agarose is used for preparative and analytical separation of nucleic acids. It can be used for single cell gel electrophoresis assay. It can also be used for southern blotting. It can also be used for assessing transepithelial ionic fluxes from cultured neonatal rat semicircular canal epithelium.

Acciones bioquímicas o fisiológicas

Agarose is a polysaccharide used for resolving DNA and RNA fragments from 500 - 20,000 bp. It provides strong gel structure which assists in better handling and less breakage. It can also reexpress the differentiated collagen phenotype from dedifferentiated chondrocytes during agarose gel culture.

Envase

Packaging
50, 500 g in poly bottle

Nota de análisis

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Safety Information

• Código de clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)