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127-2.5

Sigma-Aldrich

Poly-D-Lysine & Laminin (2.5 ML)

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Sinónimos:
Collagen IV from human placenta
Código UNSPSC:
12352202
NACRES:
NA.75

formulario

liquid

Nivel de calidad

caducidad

1 yr

técnicas

cell culture | mammalian: suitable

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Descripción general

Poly-D-Lysine (PDL) and Laminin solution is ideal for use in the coating protocol for cell culture surfaces. PDL Laminin coating is extensively used for neural cultures. It is known to enhance cell adhesion and cell maturation during in vitro cell culture. Poly-D-Lysine and Laminin solution is prepared in phosphate-buffered saline and sterile filtered. It is used to coat tissue culture ware to culture surface ratio of 0.25 ml/cm2. Tissue culture ware is coated at room temperature for one hour and carefully aspirate the solution. After wash two times with PBS, coated tissue culture ware may be used immediately or air-dried and stored at 4oC for up to one week.

Aplicación

Poly-D-Lysine & Laminin (2.5 ML) has been used in vitro as inductive agents to stimulate Y79 cells to express both neuronal and glial characteristics.It has been used to coat culture plates for retinal ganglion cell culture.

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

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