Merck
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14008C

Sigma-Aldrich

Serum Plus Medium Supplement

FBS γ-irradiated by SER-TAIN process

Sinónimos:
GI sera supplement, FCS, FBS, GI FBS, GI FCS, GI Serum supplement

Nivel de calidad

500

esterilidad

γ-irradiated by SER-TAIN process (FBS)
sterile; sterile-filtered

formulario

solution

enviado en

dry ice

temp. de almacenamiento

−20°C

Descripción general

SERUM PLUS contains a low level of gamma irradiated Fetal Bovine Serum (FBS) supplemented with proteins that promote nutrient transport, cell attachement, and cell growth.

Aplicación

Serum Plus is a medium supplement that can be used as a serum substitute for most cell culture applications. It has been used as a supplement in media for maintenance of bloodstream form Trypanosoma brucei Lister 427.

Características y beneficios

Benefits include less than half of the total protein concentration found in serum, reduced levels of inhibitors such as proteases and viral antibodies, and consistency of lot-to-lot performance due to greater control of the component materials.

Nota de preparación

Thawing
1. Remove the supplement bottles from the freezer and allow them to acclimate to room temperature for approximately 10 minutes.
2. Place each container in a 30 - 37 °C water bath or incubator. Excessive temperatures will degrade heat liable nutrients. If using a water bath, prevent the bottle caps from being completely submerged.
3. Gently swirl or shake the bottles every 10-15 minutes until the serum is completely thawed.
4. After thawing, use the supplement promptly. Liquid supplement may be stored refrigerated (2 - 8 °C) up to four weeks. To avoid freeze/thaw cycles or long periods of refrigeration, it is recommended that any unused supplement be immediately dispensed into small, useful aliquots and refrozen for future use.

Note:
Periodic agitation is crucial for optimum performance. If a bottle is not periodically shaken or swirrled as it thaws, gradients containing high concentrations of salts, proteins and lipids will form throughout the liquid portion and lead to the formation of crystalline or flocculent precipitates. These cryoprecipitates are not toxic to cell cultures, but they affect the appearance and consistency of each bottle.

Small amounts of cryoprecipitates are not uncommon, and will not affect product performance. Gently warming and mixing the supplement will generally allow the material to go back into solution.

Información legal

SER-TAIN is a trademark of Sigma-Aldrich Co. LLC
SERUM PLUS is a trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

Introduzca el número de lote para buscar un certificado de análisis (COA).

Certificado de origen

Introduzca el número de lote para buscar un Certificado de origen (COO).

Utilizing Chemical Genomics to Identify Cytochrome b as a Novel Drug Target for Chagas Disease.
Khare S et al.
PLoS Pathogens, 11, e1005058-e1005058 (2015)
Proteasome inhibition for treatment of leishmaniasis, Chagas disease and sleeping sickness.
Khare S et al.
Nature, 537, 229-229 (2016)
Nidhi Gupta et al.
Journal of the American Society of Nephrology : JASN, 31(9), 2065-2082 (2020-08-09)
Circulating APOL1 lyses trypanosomes, protecting against human sleeping sickness. Two common African gene variants of APOL1, G1 and G2, protect against infection by species of trypanosomes that resist wild-type APOL1. At the same time, the protection predisposes humans to CKD
Joseph Verdi et al.
Cell host & microbe, 28(1), 79-88 (2020-05-18)
Trypanosomiasis is a devastating neglected tropical disease affecting livestock and humans. Humans are susceptible to two Trypanosoma brucei subspecies but protected from other trypanosomes by circulating high-density lipoprotein (HDL) complexes called trypanosome lytic factors (TLFs) 1 and 2. TLFs contain

Artículos

Frequently Asked Questions About Gamma-Irradiated FBS (Serum) and Other Products for Cell Culture

Find answers to questions (FAQs) about fetal bovine serum (FBS) that is gamma-irradiated by the SER-TAIN™ process. Request a sample of FBS at Sigma-Aldrich.com.

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