56847

Sigma-Aldrich

Dy-549 NHS ester

for fluorescence

Empirical Formula (Hill Notation):
C37H42N3Na3O16S4
Peso molecular:
981.97

Quality Level

grade

for fluorescence

fluorescence

λex 554 nm; λem 568 nm in 0.1 M phosphate pH 7.0

suitability

corresponds for coupling to amines

storage temp.

2-8°C

Application

Dy-549 NHS ester, an amine coupling asymmetric cyanine reagent, is used for fluorescent labeling of amine groups within proteins, amino derivatized oligonucleotide and other amine containing molecules.

Certificado de Análisis

Certificado de origen

Franziska M Hamann et al.
Molecular imaging, 10(4), 258-269 (2011-04-28)
To assess the suitability of asymmetric cyanine dyes for in vivo fluoro-optical molecular imaging, a comprehensive study on the influence of the number of negatively charged sulfonate groups governing the hydrophilicity of the DY-67x family of asymmetric cyanines was performed....
Jutta Pauli et al.
Bioconjugate chemistry, 22(7), 1298-1308 (2011-05-19)
Aiming at the design of highly brilliant NIR emissive optical probes, e.g., for in vivo near-infrared fluorescence imaging (NIRF), we studied the absorption and fluorescence properties of the asymmetric cyanines Dy678, Dy681, Dy682, and Dy676 conjugated to the model antibody...
Marcia Levitus et al.
Quarterly reviews of biophysics, 44(1), 123-151 (2010-11-27)
The breakthroughs in single molecule spectroscopy of the last decade and the recent advances in super resolution microscopy have boosted the popularity of cyanine dyes in biophysical research. These applications have motivated the investigation of the reactions and relaxation processes...
Judith E Berlier et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(12), 1699-1712 (2003-11-19)
Amine-reactive N-hydroxysuccinimidyl esters of Alexa Fluor fluorescent dyes with principal absorption maxima at about 555 nm, 633 nm, 647 nm, 660 nm, 680 nm, 700 nm, and 750 nm were conjugated to antibodies and other selected proteins. These conjugates were...

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