Merck

62197

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−Atto 488 antibody produced in goat

~1 mg/mL

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NACRES:
NA.46

origen biológico

goat

Nivel de calidad

conjugado

Atto 488 conjugate

forma del anticuerpo

affinity isolated antibody

antibody product type

secondary antibodies

clon

polyclonal

formulario

liquid

contiene

50% glycerol as stabilizer

species reactivity

mouse

concentración

~1 mg/mL

technique(s)

immunocytochemistry: 1:500
immunofluorescence: 1:400

fluorescencia

λex 500 nm; λem 522 nm in PBS

enviado en

wet ice

temp. de almacenamiento

−20°C

target post-translational modification

unmodified

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1 of 4

Este artículo
A1047A4656A6531
conjugate

Atto 488 conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

agarose conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

liquid

form

buffered aqueous solution

form

buffered aqueous solution

form

suspension

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

Descripción general

IgG is the most abundant antibody in human serum. It is secreted by B cells andis found in blood. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, that is linked together by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Anti-Mouse IgG (whole molecule)-Atto 488 antibody is specific for mouse IgG. The product is purified from goat anti-mouse IgG antiserum to remove all goat serum proteins, including immunoglobulins that do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity.

Inmunógeno

purified mouse IgG.

Aplicación

Anti-Mouse IgG (whole molecule)?Atto 488 antibody produced in goat has been used:
  • in immunofluorescence confocal microscopic assay for p65 localization
  • in immunohistochemical analysis
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Acciones bioquímicas o fisiológicas

IgG facilitates transplacental transport and participates in the secondary immune response. It has the longest serum half life. It might be linked to allergy. IgG initiates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC).

Forma física

Solution in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, andcontaining 5 mM sodium azide as a preservative.

Nota de análisis

unconjugated dye ≤5% of total fluorescence

Información legal

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves


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Carlo Sala Frigerio et al.
Cell reports, 27(4), 1293-1306 (2019-04-25)
Gene expression profiles of more than 10,000 individual microglial cells isolated from cortex and hippocampus of male and female AppNL-G-F mice over time demonstrate that progressive amyloid-β accumulation accelerates two main activated microglia states that are also present during normal
Jan Andreas Ruland et al.
Nature communications, 12(1), 6211-6211 (2021-10-29)
Ribosomal biogenesis has been studied by biochemical, genetic and electron microscopic approaches, but live cell data on the in vivo kinetics are still missing. Here we analyse the export kinetics of the large ribosomal subunit (pre-60S particle) through single NPCs
Sumeda Nandadasa et al.
PloS one, 7(6), e38756-e38756 (2012-06-22)
Alpha (α)-E-catenin is a component of the cadherin complex, and has long been thought to provide a link between cell surface cadherins and the actin skeleton. More recently, it has also been implicated in mechano-sensing, and in the control of
Arnold Garbiec et al.
Arthropod structure & development, 41(2), 165-176 (2012-02-04)
Mechanisms that underlie differentiation and diversification of the ovarian follicular epithelium in insects have been best characterized in a fruit fly, Drosophila melanogaster. Recent comparative analyses have shown that dipterans evolved a common, specific system of early patterning of their
Emily J Reedich et al.
Journal of neuroscience research, 100(10), 1951-1966 (2022-07-16)
Spastic cerebral palsy (CP) is a movement disorder marked by hypertonia and hyperreflexia; the most prevalent comorbidity is pain. Since spinal nociceptive afferents contribute to both the sensation of painful stimuli as well as reflex circuits involved in movement, we

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