Merck
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79760

Sigma-Aldrich

Phthaldialdehyde

for fluorescence, ≥99.0% (HPLC)

Synonym(s):
o-Phthalaldehyde, o-Phthalic dicarboxaldehyde, o-Phthalic dicarboxaldehyde, OPA, Benzene-1,2-dicarboxaldehyde, o-Phthalaldehyde
Empirical Formula (Hill Notation):
C8H6O2
CAS Number:
Molecular Weight:
134.13
Beilstein:
878317
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.32

Nivel de calidad

100

grado

for fluorescence

ensayo

≥99.0% (HPLC)

formulario

solid

impurezas

≤0.5% phthalic acid

mp

54-56 °C

fluorescencia

λex 334 nm; λem 455 nm (Thiol Adduct)
λex 340 nm; λem 450 nm in reaction buffer (with glycine)

temp. de almacenamiento

2-8°C

SMILES string

O=Cc1ccccc1C=O

InChI

1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H

InChI key

ZWLUXSQADUDCSB-UHFFFAOYSA-N

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Descripción general

Phthaldialdehyde, also known as o-Phthalaldehyde or OPA, is a pre-column (HPLC) derivatization reagent. OPA reacts with primary amino acids (AA) except cystine to form highly fluorescent adducts. The fluorescence of the derivatives is monitored at excitation and emission wavelengths of 345nm and 455nm, respectively. The HPLC technique efficiently separates the OPA-AA derivatives.

Aplicación

Phthaldialdehyde or OPA determines amino acids in proteins from animal tissue and food samples. Quantification of amino acids adducts of tyramine and phenylethylamine produced by lactic acid bacteria used OPA as the pre-column derivative reagent for HPLC. Intracellular forms of glutathione (GSH/GSSG) are investigated using o-Phthalaldehyde as the pre-column fluorescent agent.
For precolumn derivatization of amino acids for HPLC separation. For flow cytometric measurements of protein thiol groups.

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Punto de inflamabilidad F

269.6 °F - closed cup

Punto de inflamabilidad C

132 °C - closed cup

Equipo de protección personal

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificate of Analysis

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Certificate of Origin

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