Merck
Todas las fotos(3)

87748

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

≥98.0% (NT)

Sinónimos:
TMB substrate, TMB, Sure Blue TMB, BM blue, TMB Blotting Plus
Fórmula lineal:
[-C6H2(CH3)2-4-NH2]2
Número de CAS:
Peso molecular:
240.34
Beilstein:
2808541
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.83

Nivel de calidad

300

ensayo

≥98.0% (NT)

formulario

powder

technique(s)

UV/Vis spectroscopy: suitable

mp

166-171 °C
168-171 °C (lit.)

temp. de almacenamiento

2-8°C

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

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Aplicación

3,3′,5,5′-Tetramethylbenzidine has been used as a substrate for peroxidase in:
  • enzyme-immunoassay (EIA)
  • indirect competitive enzyme-linked immunosorbent assay (ELISA)
  • sandwich enzyme-linked immunosorbent assay

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificado de Análisis

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Certificado de origen

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