91228

Trichloroacetic acid

BioUltra, ≥99.5% (T)

• Synonym(s): TCA
• Linear Formula: Cl₃CCOOH
• CAS Number: 76-03-9
• Molecular Weight: 163.39
• Beilstein: 970119
• Número de EC: 200-927-2
• Número MDL: MFCD00004177
• ID de la sustancia en PubChem: 57653428
• NACRES: NA.25

Properties

• Nivel de calidad: 200
• densidad de vapor: <1 (vs air)
• presión de vapor: 1 mmHg ( 51 °C)
• línea de producto: BioUltra
• ensayo: ≥99.5% (T)
• formulario: crystals; flakes
• impurezas: insoluble matter, passes filter test
• residuo de ign.: ≤0.05%
• índice de refracción: n20/D 1.62 (lit.)
• pH: <1.0 (25 °C, 0.5 M in H₂O)
• bp: 196 °C (lit.)
• mp: 54-58 °C (lit.)
• solubilidad: H₂O: 0.5 M at 20 °C, clear, colorless
• densidad: 1.62 g/mL at 25 °C (lit.)
• trazas de anión: chloride (Cl^-): ≤30 mg/kg; nitrate (NO₃^-): ≤20 mg/kg; phosphate (PO₄^3-): ≤10 mg/kg; sulfate (SO₄^2-): ≤100 mg/kg
• trazas de catión: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.5 M in H₂O
• SMILES string: OC(=O)C(Cl)(Cl)Cl
• Absorción UV: λ: 260 nm A_max: 1.60; λ: 280 nm A_max: 0.15
• InChI: 1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)
• InChI key: YNJBWRMUSHSURL-UHFFFAOYSA-N

Description

Descripción general

Trichloroacetic acid (TCA) is derived from Trichloroethylene (TCE) metabolism. It is used as an acid decalcifying agent. TCA is used as a fixative for nuclear staining and protein precipitation.

Aplicación

Trichloroacetic acid (TCA) has been used:

• to determine its effect on avian embryos during an exposure window encompassing cardiac specification
• as a cell fixative
• to terminate the O-acetylserine (thiol)lyase (OASTL) enzymatic assay
• for the precipitation of acidic ribosomal proteins (RPs)

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

Otras notas

Concentration of protein by quantitative precipitation; Cytochemical demonstration of DNA with a thiazine dye

Safety Information

• pictogramas: GHS05,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H314 - H410
• Consejos de prudencia: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Clasificaciones de peligro: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
• Código de clase de almacenamiento: 8B - Non-combustible, corrosive hazardous materials
• WGK: WGK 2
• Punto de inflamabilidad F: 235.4 °F - closed cup
• Punto de inflamabilidad C: > 113 °C - closed cup
• Equipo de protección personal: Eyeshields, Faceshields, Gloves, type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges