Merck
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91228

Sigma-Aldrich

Trichloroacetic acid

BioUltra, ≥99.5% (T)

Synonym(s):
TCA
Linear Formula:
Cl3CCOOH
CAS Number:
Molecular Weight:
163.39
Beilstein:
970119
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.25

Nivel de calidad

200

densidad de vapor

<1 (vs air)

presión de vapor

1 mmHg ( 51 °C)

línea de producto

BioUltra

ensayo

≥99.5% (T)

formulario

crystals
flakes

impurezas

insoluble matter, passes filter test

residuo de ign.

≤0.05%

índice de refracción

n20/D 1.62 (lit.)

pH

<1.0 (25 °C, 0.5 M in H2O)

bp

196 °C (lit.)

mp

54-58 °C (lit.)

solubilidad

H2O: 0.5 M at 20 °C, clear, colorless

densidad

1.62 g/mL at 25 °C (lit.)

trazas de anión

chloride (Cl-): ≤30 mg/kg
nitrate (NO3-): ≤20 mg/kg
phosphate (PO43-): ≤10 mg/kg
sulfate (SO42-): ≤100 mg/kg

trazas de catión

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.5 M in H2O

SMILES string

OC(=O)C(Cl)(Cl)Cl

Absorción UV

λ: 260 nm Amax: 1.60
λ: 280 nm Amax: 0.15

InChI

1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)

InChI key

YNJBWRMUSHSURL-UHFFFAOYSA-N

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Descripción general

Trichloroacetic acid (TCA) is derived from Trichloroethylene (TCE) metabolism. It is used as an acid decalcifying agent. TCA is used as a fixative for nuclear staining and protein precipitation.

Aplicación

Trichloroacetic acid (TCA) has been used:
  • to determine its effect on avian embryos during an exposure window encompassing cardiac specification
  • as a cell fixative
  • to terminate the O-acetylserine (thiol)lyase (OASTL) enzymatic assay
  • for the precipitation of acidic ribosomal proteins (RPs)

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

Otras notas

Concentration of protein by quantitative precipitation; Cytochemical demonstration of DNA with a thiazine dye

pictogramas

CorrosionEnvironment

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A

Código de clase de almacenamiento

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Punto de inflamabilidad F

235.4 °F - closed cup

Punto de inflamabilidad C

> 113 °C - closed cup

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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