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93352

Sigma-Aldrich

Trizma® base

≥99.0% (T)

Sinónimos:
Tris base, Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris(hydroxymethyl)aminomethane
Fórmula lineal:
NH2C(CH2OH)3
Número de CAS:
Peso molecular:
121.14
Beilstein:
741883
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.25

Nivel de calidad

200

descripción

aminopeptidase substrate

línea de producto

BioChemika

ensayo

≥99.0% (T)

formulario

crystalline

pérdida

≤1% loss on drying, 110 °C

pH

10.5-12.0(4 m in water, 25 °C)

intervalo de pH útil

7 - 9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)
168-172 °C

solubilidad

H2O: 1 M at 20 °C, clear, colorless

trazas de anión

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

trazas de catión

Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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Descripción general

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Aplicación

Trizma® base was used as buffer for the following studies:
  • Electrophoretic transfer for the specific identification of isozymes of starch debranching enzyme, α-amylase and 9-amylase.
  • Electrophoretic separation of lipoproteins in polyacrylamide gels.
  • Preparation of TRIS buffer having pH 8.
It may be used to compose DN buffer for DNA nick-end labeling of tissue sections.

Otras notas

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Información legal

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

Certificado de Análisis

Certificado de origen

Electrophoretic separation of serum lipoproteins in polyacrylamide gel.
C S Frings et al.
Clinical chemistry, 17(2), 111-114 (1971-02-01)
J M Isner et al.
Circulation, 91(11), 2703-2711 (1995-06-01)
Apoptosis has been recognized in normal, including rapidly proliferating, cell populations and is inferred to be potentially responsible for the maintenance of stable cell numbers in tissues with various degrees of proliferative activity. Previous studies performed in rats indicated that
Cyril Lafon et al.
Ultrasound in medicine & biology, 31(10), 1383-1389 (2005-10-15)
An optically transparent phantom was developed for use in high-intensity focused ultrasound (US), or HIFU, dosimetry studies. The phantom is composed of polyacrylamide hydrogel, embedded with bovine serum albumin (BSA) that becomes optically opaque when denatured. Acoustic and optical properties
G Kakefuda et al.
Plant physiology, 75(1), 278-280 (1984-05-01)
An electrophoretic transfer technique was developed for the specific identification of isozymes of starch debranching enzyme, alpha-amylase, and beta-amylase. Amylolytic enzymes are separated by native polyacrylamide slab gel electrophoresis and proteins in gels are electrophoretically transferred through starch-containing polyacrylamide gels.
Marcel Kuiper et al.
Biotechnology progress, 35(4), e2821-e2821 (2019-04-16)
Perfusion is a cell culture mode that is gaining popularity for the manufacture of monoclonal antibodies and their derivatives. The cell culture media supporting perfusion culture need to support higher cell densities than those used in fed-batch culture. Therefore, when

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