Merck
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93362

Sigma-Aldrich

Trizma® base

BioUltra, for molecular biology, ≥99.8% (T)

Sinónimos:
Tris base, Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris(hydroxymethyl)aminomethane
Fórmula lineal:
NH2C(CH2OH)3
Número de CAS:
Peso molecular:
121.14
Beilstein:
741883
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.25

Nivel de calidad

200

grado

for molecular biology

descripción

aminopeptidase substrate

línea de producto

BioUltra

ensayo

≥99.8% (T)

formulario

crystalline

impurezas

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

residuo de ign.

≤0.01% (as SO4)

pérdida

≤0.5% loss on drying, 110 °C

color

colorless to white

pH

10.5-12.0 (25 °C, 4 M in H2O)

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)
168-172 °C

solubilidad

H2O: 4 M at 20 °C, clear, colorless

trazas de anión

chloride (Cl-): ≤20 mg/kg
sulfate (SO42-): ≤5 mg/kg

trazas de catión

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES string

NC(CO)(CO)CO

λ

4 M in H2O

Absorción UV

λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.08

idoneidad

in accordance for luminescence

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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Descripción general

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

The BioChemika quality is for the usual biochemical applications. The products designated as BioChemika Ultra grade are suitable for different applications like purification, precipitation, crystallization and other applications which require tight control of elemental content. Trace elemental analyses have been performed for all qualities. The molecular biology qualities are also tested for absence of nucleases and the luminescence quality spectroscopic tests are performed.

Aplicación

Trizma has been used:
  • In the dissection medium and culture medium of Hippocampal organotypic tissue cultures
  • As a component of lysis buffer before immunoprecipitation and immunoblotting
  • As a component of harvest buffer, lysis buffer, ChIP dilution buffer, and wash buffer in chromatin immunoprecipitation method for studying protein-DNA binding
  • As a component of mixed buffer formulations
  • As a component of NuPAGE loading buffer for SDS-PAGE and as a component of transfer buffer for Western blotting
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma base is used as a general reagent for the preparation of all types of Tris buffers.

Otras notas

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Easily compare specifications for Trizma products with the Trizma specification table.
Running buffer component in non-denaturing agarose gel electrophoresis; In non-denaturing and urea-denaturing polyacrylamide gel electrophoresis; DNA digest analysis with capillary electrophoresis.

Información legal

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

Certificado de Análisis

Introduzca el número de lote para buscar un certificado de análisis (COA).

Certificado de origen

Introduzca el número de lote para buscar un Certificado de origen (COO).

A new method allowing long-term potentiation recordings in hippocampal organotypic slices.
Paci P, et al.
Brain and Behavior, 7(5), e00692-e00692 (2017)
Differentiation of ruminant transmissible spongiform encephalopathy isolate types, including bovine spongiform encephalopathy and CH1641 scrapie.
Jacobs JG, et al.
The Journal of General Virology, 92(1), 222-232 (2011)
A fast, efficient chromatin immunoprecipitation method for studying protein-DNA binding in Arabidopsis mesophyll protoplasts.
Plant methods, 13(1), 42-42 (2017)
In vitro Detection of S-acylation on Recombinant Proteins via the Biotin-Switch Technique.
Qi D and Innes RW
Bio-protocol, 4(22) (2014)
G G Nahas et al.
Drugs, 55(2), 191-224 (1998-03-20)
THAM (trometamol; tris-hydroxymethyl aminomethane) is a biologically inert amino alcohol of low toxicity, which buffers carbon dioxide and acids in vitro and in vivo. At 37 degrees C, the pK (the pH at which the weak conjugate acid or base

Protocolos

Restriction Enzyme Cloning Manual Buffer Recipes

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

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