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Merck

96631

Sigma-Aldrich

Bis(2,2′-bipyridine)-4′-methyl-4-carboxybipyridine-ruthenium N-succinimidyl ester-bis(hexafluorophosphate)

BioReagent, suitable for fluorescence

Sinónimos:

Ru(bpy)2(mcbpy-O-Su-ester)(PF6)2

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About This Item

Fórmula empírica (notación de Hill):
C36H29F12N7O4P2Ru
Número de CAS:
Peso molecular:
1014.66
MDL number:
UNSPSC Code:
12352108
PubChem Substance ID:
NACRES:
NA.32

product line

BioReagent

Quality Level

form

powder

fluorescence

λex 458 nm; λem 628 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

shipped in

dry ice

storage temp.

−20°C

SMILES string

[Ru++].F[P-](F)(F)(F)(F)F.F[P-](F)(F)(F)(F)F.c1ccc(nc1)-c2ccccn2.c3ccc(nc3)-c4ccccn4.Cc5ccnc(c5)-c6cc(ccn6)C(=O)ON7C(=O)CCC7=O

InChI

1S/C16H13N3O4.2C10H8N2.2F6P.Ru/c1-10-4-6-17-12(8-10)13-9-11(5-7-18-13)16(22)23-19-14(20)2-3-15(19)21;2*1-3-7-11-9(5-1)10-6-2-4-8-12-10;2*1-7(2,3,4,5)6;/h4-9H,2-3H2,1H3;2*1-8H;;;/q;;;2*-1;+2

InChI key

UQJZJWDQEYXDCR-UHFFFAOYSA-N

Application

Bis(2,2′-bipyridine)-4′-methyl-4-carboxybipyridine-ruthenium N-succinimidyl ester-bis(hexafluorophosphate) has been used:
  • as a component in the preparation of ruthenium labelling agent solution (RuC) as an extrinsic fluorophore
  • to stain protein in mass cytometry
  • in the synthesis of ruthenium (Ru) gelatin
  • in the preparation of streptavidin-Ru(bpy)32+ mixture involved in aptamer-magnetic bead electrochemiluminescence (ECL) sandwich assays

Activated ester of ruthenium complex for acylation of amino acid side chain amines.

Features and Benefits

This dye has a long fluorescence lifetime (near 500 nanoseconds) with high photostability, good water solubility, a lack of dye-dye interactions and large Stokes′ shifts.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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CellCycleTRACER accounts for cell cycle and volume in mass cytometry data
Rapsomaniki M, et al.
Nature Communications, 9(1), 632-632 (2018)
Fluorometric enzymatic autoindicating biosensor for H2O2 determination based on modified catalase
Ortega E, et al.
Biosensors And Bioelectronics, 41, 150-156 (2013)
Bernd Geisser et al.
Inorganic chemistry, 38(9), 2030-2037 (2001-10-24)
The pH-dependent photophysical properties of a series of polypyridyl ruthenium-substituted amino acids were investigated by steady-state and time-resolved luminescence spectroscopy. [H(3)N-DAPA(Rub(2)m)-OH](PF(6))(3) (1), [H(3)N-DABA(Rub(2)m)-OH](PF(6))(3) (2), [H(3)N-Orn(Rub(2)m)-OH](PF(6))(3) (3), and [H(3)N-Lys(Rub(2)m)-OH](PF(6))(3) (4) were obtained by formation of an amide link between the omega-NH(2)
B M Peek et al.
International journal of peptide and protein research, 38(2), 114-123 (1991-08-01)
Boc-L-Lysine derivatives and lysine-containing peptides bearing the electron donor 10H-phenothiazine (PTZ) or the redox chromophore tris(2,2'-bipyridine)ruthenium(II) dication ([Rub3]2+, where b is 2,2'-bipyridine) have been synthesized and characterized. SeO2 oxidation (53% yield) of 4,4'-dimethyl-2,2'-bipyridine, Ag2O oxidation (85% yield) of the monoaldehyde
Preliminary development of a DNA aptamer-magnetic bead capture electrochemiluminescence sandwich assay for brain natriuretic peptide
Bruno J, et al.
Microchemical Journal, Devoted to the Application of Microtechniques in All Branches of Science, 115, 32-38 (2014)

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