A0157

Sigma-Aldrich

Ascorbate Oxidase from Cucurbita sp.

lyophilized powder, 1,000-3,000 units/mg protein

Sinónimos:
Ascorbase, L-Ascorbate:oxygen oxidoreductase
Número de CAS:
Comisión internacional de enzimas:
EC Number:
MDL number:
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

1,000-3,000 units/mg protein

composition

Protein, ~10% Lowry

storage temp.

2-8°C

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General description

Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.

Application

Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Ascorbate oxidase, from Sigma, has been used in ascorbic acid assays to study the heat stress response of Arabidopsis .
Ascorbate Oxidase from Cucurbita sp. has been used:
  • as a supplement in the culture medium for differentiation into osteoblasts
  • to oxidize ascorbic acid producing monodehydroascorbate in monodehydroascorbate reductase assay
  • in determining ascorbate (AsA) and dehydroascorbate (DHA) concentrations

Packaging

100, 250, 1000 units in poly bottle

Biochem/physiol Actions

Ascorbate oxidase (AO) participates in cell growth by regulating reduction/oxidation (redox) of the apoplast. This enzyme can synthesize the oxidative molecule dehydroascorbate acid (DHA) in the apoplast. It is also involved in cell elongation and enlargement development.
Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. It is highly specific for L-ascorbic acid and a few analogs. Ascorbate oxidase exists as a dimer and has a molecular weight of approximately 140 kDa.

Unit Definition

One unit will oxidize 1.0 μmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25 °C.

Physical form

Lyophilized powder containing buffers and sucrose as stabilizer.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Cotton ascorbate oxidase promotes cell growth in cultured tobacco bright yellow-2 cells through generation of apoplast oxidation
Li R, et al.
International Journal of Molecular Sciences, 18(7), 1346-1346 (2017)
Manuel A Matamoros et al.
Journal of experimental botany, 61(1), 87-97 (2009-10-14)
In plant cells, antioxidants keep reactive oxygen species at low concentrations, avoiding oxidative damage while allowing them to play crucial functions in signal transduction. However, little is known about the role of antioxidants during fruit maturation, especially in legumes. Snap...
Ming Liu et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 27(5), 477-477 (2011-05-12)
An amperometric L-ascorbic acid (AA) biosensor fabricated by immobilizing ascorbate oxidase (AO) in poly(3,4-ethylenedioxythiophene) (PEDOT) and multi-walled carbon nanotubes (MWCNTs) composite films was reported for the first time. The entrapment of AO in PEDOT/MWCNTs composite films was performed during an...
Irina I Panchuk et al.
Plant physiology, 129(2), 838-853 (2002-06-18)
To find evidence for a connection between heat stress response, oxidative stress, and common stress tolerance, we studied the effects of elevated growth temperatures and heat stress on the activity and expression of ascorbate peroxidase (APX). We compared wild-type Arabidopsis...
Fiona L Goggin et al.
BioEssays : news and reviews in molecular, cellular and developmental biology, 32(9), 777-790 (2010-07-29)
Analysis of a diverse cross-sample of plant-insect interactions suggests that the abundance of vitamin C (L-ascorbic acid, ascorbate or AsA) in plants influences their susceptibility to insect feeding. These effects may be mediated by AsAs roles as an essential dietary...

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