Merck
Todas las fotos(2)

A0545

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

affinity isolated antibody

Sinónimos:
Anti Rabbit Igg, Anti Rabbit, Anti Rabbit Antibody, Goat Anti Rabbit Igg, Goat Anti Rabbit IgG Antibody - Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat, Anti-Rabbit Igg
Número MDL:
NACRES:
NA.46

origen biológico

goat

conjugado

peroxidase conjugate

forma del anticuerpo

affinity isolated antibody

antibody product type

secondary antibodies

clon

polyclonal

species reactivity

rabbit

should not react with

human

technique(s)

direct ELISA: 1:30,000 using using 5 μg/ml of rabbit IgG for the coating and OPD substrate
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot (chemiluminescent): 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg/mL)

enviado en

dry ice

temp. de almacenamiento

−20°C

¿Está buscando productos similares? Visit Guía de comparación de productos

Descripción general

Goat anti-rabbit IgG-peroxidase conjugate associates with all rabbit Igs. The conjugate shows no reaction with human IgG by immunoelectrophoresis (IEP). Furthermore, the antibody is adsorbed with human IgG to reduce background staining with human samples.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy disulfide bridge. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Especificidad

Specificity of the Anti-Rabbit IgG-Peroxidase is determined by immunoelectrophoresis (IEP) using normal rabbit serum and rabbit IgG. The conjugate shows no reaction with human IgG by IEP.

Inmunógeno

Purified rabbit IgG

Aplicación

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody has been used in western blotting , immunofluorescence staining , immunochemistry and immunoprecipitations.
Goat anti-rabbit IgG conjugated to peroxidase can be used for direct ELISA and immunohistochemistry.
Peroxidase-conjugated goat anti-rabbit IgG antibody was used for western blot analysis of Cos 7 cell lysates at a 1:2000 dilution and yeast lysates following protein carbonylation assays at a dilution of 1:5000.
Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in goat has been used as a secondary antibody during immunofluorescence staining.
Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.

Acciones bioquímicas o fisiológicas

IgGs (immunoglobulins) modulate several immune responses.It mainly helps in immune defense. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Nota de preparación

Prepared by two-step glutaraldehyde method.

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Skin Sens. 1

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

Introduzca el número de lote para buscar un certificado de análisis (COA).

Certificado de origen

Introduzca el número de lote para buscar un Certificado de origen (COO).

Teresa Almeida et al.
Molecular biology of the cell, 19(3), 865-876 (2007-12-29)
The inositolphosphosphingolipid phospholipase C (Isc1p) of Saccharomyces cerevisiae belongs to the family of neutral sphingomyelinases that generates the bioactive sphingolipid ceramide. In this work the role of Isc1p in oxidative stress resistance and chronological lifespan was investigated. Loss of Isc1p
V Lila Koumandou et al.
Journal of cell science, 124(Pt 9), 1496-1509 (2011-04-20)
Intracellular trafficking and protein sorting are mediated by various protein complexes, with the retromer complex being primarily involved in retrograde traffic from the endosome or lysosome to the Golgi complex. Here, comparative genomics, cell biology and phylogenetics were used to
Yu Xiao et al.
Nature, 572(7768), 270-274 (2019-07-11)
Receptor kinases of the Catharanthus roseus RLK1-like (CrRLK1L) family have emerged as important regulators of plant reproduction, growth and responses to the environment1. Endogenous RAPID ALKALINIZATION FACTOR (RALF) peptides2 have previously been proposed as ligands for several members of the
The transcription factor grainyhead-like 2 regulates the molecular composition of the epithelial apical junctional complex
Werth M, et al.
Development, 137(22), 3835-3845 (2010)
Kristen J Nowak et al.
The Journal of cell biology, 185(5), 903-915 (2009-05-27)
Skeletal muscle alpha-actin (ACTA1) is the major actin in postnatal skeletal muscle. Mutations of ACTA1 cause mostly fatal congenital myopathies. Cardiac alpha-actin (ACTC) is the major striated actin in adult heart and fetal skeletal muscle. It is unknown why ACTC

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico