A1287

5-Azacytidine

Hybri-Max^™, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

• Synonym(s): 4-Amino-1-(β-D-ribofuranosyl)-1,3,5-triazin-2(1H)-one, Ladakamycin
• Empirical Formula (Hill Notation): C₈H₁₂N₄O₅
• CAS Number: 320-67-2
• Molecular Weight: 244.20
• Beilstein: 620461
• Número de EC: 206-280-2
• Número MDL: MFCD00006539
• ID de la sustancia en PubChem: 24890525
• NACRES: NA.75

Properties

• Nivel de calidad: 200
• origen biológico: synthetic (organic)
• grado: Hybri-Max^™
• esterilidad: γ-irradiated
• línea de producto: BioXtra
• formulario: lyophilized powder
• technique(s): cell culture | hybridoma: suitable
• impurezas: endotoxin, tested
• mp: 226-232 °C (dec.) (lit.)
• solubilidad: acetic acid: water (1:1): 5 mg/mL, clear, colorless
• espectro de actividad antibiótica: viruses
• Modo de acción: DNA synthesis | interferes
• temp. de almacenamiento: −20°C
• SMILES string: NC1=NC(=O)N(C=N1)[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O
• InChI: 1S/C8H12N4O5/c9-7-10-2-12(8(16)11-7)6-5(15)4(14)3(1-13)17-6/h2-6,13-15H,1H2,(H2,9,11,16)/t3-,4-,5-,6-/m1/s1
• InChI key: NMUSYJAQQFHJEW-KVTDHHQDSA-N
• Gene Information: human ... DNMT1(1786) , DNMT3A(1788)

Description

Descripción general

Chemical structure: nucleoside

Aplicación

5-Azacytidine may be used to induce in vitro cardiomyogenic differentiation of adult human bone marrow mesenchymal stem cells. It may also be used to study the effects of 5-Azacytidine on the immune regulatory myeloid-derived suppressor cells (MDSCs) and regulatory T cells (Tregs).

Used to study the effects of the DNA incorporated molecule on DNA methylation processes.

Acciones bioquímicas o fisiológicas

5-Azacytidine is a deoxycytidine analog and a demethylating agent. It acts as a potential antineoplastic agent for acute myelogenous leukemia. 5-Azacytidine activates repressed genes by inhibiting DNA methylation. 5-Azacytidine also affects protein synthesis by altering the RNA function and stability.

A potent growth inhibitor and cytotoxic agent; inhibits DNA methyltransferase, an important regulatory mechanism of gene expression, gene activation and silencing.

Reconstitución

Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500ml medium. Final working concentration: 10 μM 5-azacytidine.

Información legal

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• pictogramas: GHS07,GHS08
• Palabra de señalización: Danger
• Frases de peligro: H302 - H360D
• Consejos de prudencia: P201 - P301 + P312 + P330 - P308 + P313
• Clasificaciones de peligro: Acute Tox. 4 Oral - Repr. 1B
• Código de clase de almacenamiento: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Equipo de protección personal: Eyeshields, Gloves, type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges