A2951

Sigma-Aldrich

Monoclonal Anti-Alkaline Phosphatase, Human Placental antibody produced in mouse

clone 8B6, ascites fluid

Sinónimos:
PLAP, Anti-Phosphatase, Alkaline, Human Placental, Monoclonal Anti-Alkaline Phosphatase, Human Placental
MDL number:
NACRES:
NA.46

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

8B6, monoclonal

contains

15 mM sodium azide

species reactivity

human

application(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:4,000 using human placenta
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable using whole cells
radioimmunoassay: suitable using radio-immunolocalization of xenografts of human cell lines expressing PLAP in nude mice2
western blot: suitable

isotype

IgG2a

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ALPP(250)

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General description

Monoclonal Anti-Human Placental Alkaline Phosphatase (mouse IgG2a isotype) is derived from the 8B6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Placental alkaline phosphatase (PLAP) or ALPP is a phosphomonoesterase. It is expressed in the placenta and endometrium. The PLAP gene is localized on human chromosome 2q37.1. Small amounts of hPLAP are found in the endocervix, fallopian tubes and lung. Very small amounts of heat-stable AP resembling hPLAP (hPLAPlike AP) are expressed in the testis, thymus and in rare colon epithelial cells.
In SDS gels, the product reacts with both Regan and Nagao isozymes of human placental alkaline phosphatase (hPLAP, 130 kDa, 67/130 kDa). By RIA, the antibody binds to hPLAP with an affinity constant of 5 × 109 LM-1. It does not react with PLAP-like enzymes.

Immunogen

Human epidermoid carcinoma cell line expressing placental alkaline phosphatase.

Application

Monoclonal Anti-Alkaline Phosphatase, Human Placental antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • radio-immunolocalization
  • western blot
  • immunofluorescence
  • immunohistochemistry

Cultured pancreatic islet cells were fixed with cold 4% paraformaldhyde at room temperature for 10 minutes and incubated with mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody at 1:500. Mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody was used to detect a unique molecular probe that consisted of the FGFR extracellular domain expressed as a chimera with human PLAP.
Anti-Alkaline phosphatase human placental antibody coupled to agarose was packed in a column and used to purify alkaline-phosphatase conjugated FGF receptor out of conditioned media from COS-7 cells. Column was washed with 1M NaCl.

Biochem/physiol Actions

Placental alkaline phosphatase (PLAP) or ALPP is a hydrolase, which is involved in the hydrolysis of phosphomonoesters. It has been shown to be a biomarker for ovarian cancer. The protein is expressed in carcinomas such as ovarian adenocarcinoma, dysgerminoma, serous cystadenocarcinoma and undifferentiated carcinoma. Although hPLAP has been analyzed as a potential tumor marker, it has not been widely used in the routine clinical laboratory because of its low overall specificity due to heat-inactivation, cross-reactivity with intestinal AP and L-phenylalanine sensitivity. The use of monoclonal antibodies as reagents has greatly improved the sensitivity, reproducibility and accuracy of the tests used to distinguish placental isozymes from their tumor cell counterparts. Thus, monoclonal anti-alkaline phosphatase may be useful as diagnostic tools for tumor detection.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis

Certificado de origen

Cloverleaf skull anomaly and de novo trisomy 4p
de Brasi D, et al.
Journal of medical Genetics, 36(5), 422-424 (1999)
Refined structures of placental alkaline phosphatase show a consistent pattern of interactions at the peripheral site
Stec B, et al.
Acta Crystallographica Section F, Structural Biology and Crystallization Communications, 66(Pt), 8-8 (2010)
A human monoclonal antibody specific to placental alkaline phosphatase, a marker of ovarian cancer
Ravenni N, et al.
MAbs, 6(1), 86-94 (2019)
Isolation and purification of human placental plasma membranes from normal and pre-eclamptic pregnancies. A comparative study
Jimenez V, et al.
Placenta, 25(5), 422-437 (2004)
Human placental alkaline phosphatase-mediated hydrolysis correlates tightly with the electrostatic contribution from tail group
Yang Y, et al.
Chemical Biology & Drug Design, 78(6), 923-931 (2011)

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