A3699

Sigma-Aldrich

Acrylamide/Bis-acrylamide, 30% solution

BioReagent, suitable for electrophoresis, 37.5:1

MDL number:
PubChem Substance ID:
NACRES:
NA.25

sterility

0.2 μm filtered

Quality Level

product line

BioReagent

feed ratio

37.5:1

application(s)

electrophoresis: suitable

suitability

suitable for electrophoresis

storage temp.

2-8°C
2-8°C

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

¿Está buscando productos similares? Visit Guía de comparación de productos

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

Acrylamide/Bis-acrylamide, 30% solution has been used:
  • in the preparation of polyacrylamide gel for photobleaching experiment
  • as a constituent in the preparation of gel precursor solution and pore-gradient microgel precursor solution
  • in the preparation of polyacrylamide gels during western blot analyses

These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

pictograms

Exclamation markHealth hazard

signalword

Danger

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

UN 3426 6.1 / PGIII

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Chi-Chih Kang et al.
Analytical chemistry, 86(20), 10429-10436 (2014-09-17)
Intratumor heterogeneity remains a major obstacle to effective cancer therapy and personalized medicine. Current understanding points to differential therapeutic response among subpopulations of tumor cells as a key challenge to successful treatment. To advance our understanding of how this heterogeneity...
Light-dependent degradation of PIF3 by SCFEBF1/2 promotes a photomorphogenic response in Arabidopsis
Dong J, et al.
Current Biology, 27(16), 2420-2430 (2017)
Single cell--resolution western blotting
Kang CC, et al.
Nature Protocols, 11(8), 1508-1508 (2016)
Ana Techniqs in Biotechnology (2011)
Engineering and characterizing monomeric fluorescent proteins for live-cell imaging applications
Ai HW, et al.
Nature Protocols, 9(4), 910-910 (2014)

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico

Redes sociales

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Investigación. Desarrollo. Producción.

Somos un proveedor líder para la industria de Ciencias de la Vida con soluciones y servicios para investigación, desarrollo y producción biotecnológicos, y para desarrollo y producción de tratamientos farmacéuticos

© 2021 Merck KGaA, Darmstadt, Alemania y/o sus filiales. Todos los derechos reservados.

Queda estrictamente prohibida la reproducción sin permiso de cualquiera de los materiales de la página web.