Merck
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A3803

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, lyophilized powder, essentially fatty acid free, ≥98% (agarose gel electrophoresis)

Sinónimos:
Albumin bovine serum, Bovine albumin, BSA
Número de CAS:
Número de EC:
Número MDL:

Nivel de calidad

200

origen biológico

bovine

ensayo

≥98% (agarose gel electrophoresis)

formulario

lyophilized powder

mol peso

~66 kDa

purificado por

heat shock fractionation

envase

poly bottle of

origen

USA origin

technique(s)

cell culture | mammalian: suitable

impurezas

≤0.005% Fatty acids

pérdida

≤5.0%, Loss on drying

pH

7

solubilidad

water: soluble (40 mg/ml)

idoneidad

suitable for ELISA applications (as diluent)

Nº de acceso UniProt

temp. de almacenamiento

2-8°C

Gene Information

bovine ... ALB(280717)

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Descripción general

Bovine serum albumin (BSA) is a 66.4 kDa, water-soluble protein composed of 583 amino acids. It has a single polypeptide chain with three homologous domains formed by six α-helices. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating. BSA has been used as a model protein in various applications, including immunodiagnostic procedures, cell culture media and clinical chemistry. Serum albumins facilitates drug disposition and efficacy by maintaining osmotic blood pressure.

Aplicación

Bovine Serum Albumin has been used:
  • to prevent nonspecific adsorption of fibrin matrices to tissue culture plates
  • as a constituent in the preparation of albumin droplets
  • as a supplement in L-15 medium to increase spermatogonia number without enhancing the growth of somatic cells
  • as a standard protein to evaluate metal oxide adsorption isotherms

Among its many applications, BSA is a fatty acid (FA) carrier. Thus when BSA is used in applications like cell culture, it is important to exercise control over particular FA′s for cell culture, because specific fatty acids can affect different cell lines in a variable manner. In order to control fatty acid content in cell culture, fatty acid-free BSA is useful, for researchers to be able fatty acids specific to their cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific FA′s to use in cell culture. The use of FA-free BSA also addresses concerns about endogenous fatty acids that might be potentially present in non-FA-free BSA.

Envase

10, 50, 100 g in poly bottle

Acciones bioquímicas o fisiológicas

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Características y beneficios

  • Heat shock fractionated
  • Essentially fatty acid-free

Nota de preparación

Prepared using heat shock fractionation. Further purified to reduce fatty acid content.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificado de Análisis

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Certificado de origen

Introduzca el número de lote para buscar un Certificado de origen (COO).

The role of inertial cavitation in acoustic droplet vaporization
Fabiilli ML, et al.
IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control, 56(5), 1006-1017 (2009)
Protein adsorption onto metal oxide materials in white wine model systems
Pachova V, et al.
Journal of Food Science, 67(6), 2118-2121 (2002)
Improved in vitro culture conditions to enhance the survival, mitotic activity, and transplantability of rainbow trout type a spermatogonia
Shikina S and Yoshizaki G
Biology of Reproduction, 83(2), 268-276 (2010)
Initial investigation of acoustic droplet vaporization for occlusion in canine kidney
Zhang M, et al.
Ultrasound in Medicine & Biology, 36(10), 1691-1703 (2010)
Ninette Genster et al.
Scientific reports, 7(1), 3852-3852 (2017-06-22)
Ficolins are a family of pattern recognition molecules that are capable of activating the lectin pathway of complement. A limited number of reports have demonstrated a protective role of ficolins in animal models of infection. In addition, an immune modulatory

Artículos

Fluorescent in situ Hybridization (FISH)

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

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