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Merck

A4596

Millipore

ANTI-FLAG® M1 Agarose Affinity Gel

Sinónimos:

Anti-ddddk, Anti-dykddddk

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1 ML
MXP 13,858.00
5 ML
MXP 31,307.00
10 ML
MXP 56,769.00
25 ML
MXP 113,121.00

MXP 13,858.00


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1 ML
MXP 13,858.00
5 ML
MXP 31,307.00
10 ML
MXP 56,769.00
25 ML
MXP 113,121.00

About This Item

Código UNSPSC:
12352203
NACRES:
NA.32

MXP 13,858.00


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conjugado

agarose conjugate

Nivel de calidad

tipo de anticuerpo

primary antibodies

Formulario

suspension

isotipo

IgG12b

capacidad

≥0.6 mg/mL, gel binding capacity

temp. de almacenamiento

−20°C

Descripción general

ANTI-FLAG® M1 Agarose Affinity Gel is a purified IgG2B monoclonal antibody covalently attached to agarose.

Especificidad

Binding specificity: Free N-Terminus of FLAG sequence
N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Aplicación

For purification of N-terminal FLAG fusion proteins. Since binding is Ca2+-dependent, proteins can be eluted with a buffer containing EDTA, as well as by the standard methods using either FLAG peptide or glycine-HCl buffer, pH 3. ANTI-FLAG M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.

Affinity gel is for calcium mediated purification of N-terminal FLAG fusion proteins.

immunoprecipitation (IP): suitable

Elution - FLAG peptide, Glycine, pH 3.5 EDTA

Learn more product details in our FLAG® application portal.

Características y beneficios

  • Typically purify fusion proteins from crude lysates to single band purity in just one chromatography step.
  • Fusion protein may be eluted from affinity resin by mild elution with EDTA.
  • A solution of FLAG peptide can be used for gentle, non-denaturing elution of FLAG fusion proteins.

Forma física

Suspension of beaded agarose in 50% glycerol containing 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, 0.02% (w/v) sodium azide

Otras notas

ANTI-FLAG® M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.

Información legal

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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