A6229

Anti-AKR1C3 antibody, Mouse monoclonal

clone NP6.G6.A6, purified from hybridoma cell culture

• Sinónimos: Anti-AKR1C3
• Número MDL: MFCD06798693
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified immunoglobulin
• antibody product type: primary antibodies
• clon: NP6.G6.A6, monoclonal
• formulario: buffered aqueous solution
• mol peso: antigen ~38 kDa
• species reactivity: human
• envase: antibody small pack of 25 μL
• technique(s): immunohistochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.25-0.5 μg/mL using cytosolic fraction extract of A549 human lung carcinoma cell
• isotipo: IgG1
• Nº de acceso UniProt: P42330
• enviado en: dry ice
• temp. de almacenamiento: −20°C
• Gene Information: human ... AKR1C3(8644)

Descripción

Descripción general

Anti-AKR1C3 antibody, Mouse monoclonal (humanα - hydroxysteroid dehydrogenase, type 2,3; 17β- hydroxysteroid dehydrogenase, type 5) (mouse IgG1 isotype) is derived from the hybridoma NP6.G6. A6 produced by the fusion of mouse myeloma cells (SP20 cells) and splenocytes from BALB/c mice immunized with human AKR1C3 protein. Oxidoreduction of natural and foreign substrates is performed by three enzymes superfamilies, one of them being the AKRs (aldo-keto reductases) family. This family contains 114 proteins that are expressed in prokaryotes and eukaryotes and is divided into 14 subfamilies (AKR1-AKR14). The AKR1 family is the largest among the different fourteen families and contains the aldose reductases, aldehyde reductases, hydroxysteroid dehydrogenases, and steroid 5 β-reductases. AKR1Cs are expressed in many tissues.

Inmunógeno

human AKR1C3 protein.

Aplicación

Anti-AKR1C3 antibody, Mouse monoclonal has been used in

• western blotting
• immunohistochemistry
• enzyme linked immunoassay (ELISA).

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Immunohistochemistry on human placentas were performed using monoclonal mouse anti-AKR1C3 at a dilution of 1:100 or western blot at 1:500.

Mouse monoclonal clone NP6.G6.A6 anti-AKR1C3 antibody used to tag aldo-keto reductase 1C3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of aldo-keto reductase 1C3 in the regulation of the androgen 5α-dihydrotestosterone (DHT) and 3α-diol compound levels in vivo.

Acciones bioquímicas o fisiológicas

In humans AKR1C3 (aldo-keto reductase family 1 member C3) including AKR1C1,2 and 4, catalyze the reduction of the androgen 5α-dihydrotestosterone (DHT) into inactive 3β or 3α-androstane diol (3α/β-Diol). AKR1C1-4 isoforms also display 3α[17β]-hydroxysteroid oxidase activity using 3α-Diol as a substrate, in vitro. In non-small cell lung carcinoma, AKR1Cs expression is dramatically increased. Due to their product profile and discrete tissue localization, they may regulate the level of active androgens, estrogens, and progestins in target tissues.

Descripción de destino

AKR1C3, aldo-keto reductase family 1, member C3 (3-α hydroxysteroid dehydrogenase, type II), encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 2
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)