A8580

Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit

IgG fraction of antiserum, PBS suspension

• Sinónimos: Anti-Glutathione-S-Transferase, Anti-GST
• NACRES: NA.46

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: agarose conjugate
• forma del anticuerpo: IgG fraction of antiserum
• antibody product type: primary antibodies
• clon: polyclonal
• formulario: PBS suspension
• mol peso: antigen 27.5 kDa
• should not react with: porcine, bovine, human, rabbit, rat
• technique(s): ELISA: suitable; immunoprecipitation (IP): suitable
• enviado en: wet ice
• temp. de almacenamiento: 2-8°C
• Gene Information: human ... GSTP1(2950)

Descripción

Descripción general

Glutathione-S-transferases (GSTs) are dimeric proteins, which belongs to phase II detoxification enzymes family. The highly polymorphic human cytosolic GSTs are divided into six classes, such as, α, μ, ω, π, θ, and ζ.

The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

Inmunógeno

recombinant GST from Schistosoma japonicum expressed in E. coli.

Aplicación

Immunoprecipitation was performed on NIH 3T3 cells transiently transfected with GST fusion proteins. IP was performed using agarose-linked rabbit anti-GST IgG with mixing for 2 hours at 4 degrees.

Rabbit Anti-Glutathione-S-Transferase (GST)-Agarose antibody can be used for ELISA, immunoaffinity purification and immunoprecipitation assays.

Acciones bioquímicas o fisiológicas

Glutathione-S-transferases (GSTs) can catalyse the conjugation of glutathione (GSH) to various endogenous and exogenous electrophilic compounds. The π and μ classes of GSTs controls the mitogen-activated protein (MAP) kinase pathway. It can also serve as transport proteins.

Forma física

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Nota de preparación

Prepared by coupling cyanogen bromide-activated agarose at 7-8 mg antibody per mL resin volume.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable