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A8592

Sigma-Aldrich

Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

origen biológico

mouse

Nivel de calidad

200

conjugado

peroxidase conjugate

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

M2, monoclonal

formulario

buffered aqueous glycerol solution

species reactivity

all

concentración

~1 mg/mL

technique(s)

indirect ELISA: 1:20,000

isotipo

IgG1

secuencia del inmunógeno

DYKDDDDK

enviado en

wet ice

temp. de almacenamiento

−20°C

Gene Information

human ... GPX1(2876)

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Descripción general

The Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides.

Envase

0.2, 1 mg in poly bottle

Aplicación

For simple, one-step detection by immunocytochemistry, ELISA, or Western blotting. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity.
Suggested dilution for immunocytochemistry and western blotting 1:100 to 1:1000
Suggested dilution for ELISA 1:20,000

Browse additional application references in our FLAG® Literature portal.

Forma física

Solution in phosphate buffered saline containing 50% glycerol plus preservative and stabilizer

Nota de preparación

Dilute ANTI-FLAG M2-Peroxidase solution in Tris Buffered Saline (TBS): 0.05 M Tris, pH 7.4, with 0.15 M NaCl.

Información legal

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

pictogramas

Exclamation markEnvironment

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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