Merck
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A9044

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sinónimos:
Anti Mouse Antibody, Anti Mouse Hrp Sigma, Anti Mouse Hrp, Sigma Anti Mouse Hrp, Anti Mouse Antibody - Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit, Anti Mouse Igg
Número MDL:
NACRES:
NA.46

Nivel de calidad

300

origen biológico

rabbit

conjugado

peroxidase conjugate

forma del anticuerpo

IgG fraction of antiserum

antibody product type

secondary antibodies

clon

polyclonal

formulario

buffered aqueous solution

technique(s)

direct ELISA: 1:40,000 using using 5 μg/ml of mouse IgG for coating and OPD substrate
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot (chemiluminescent): 1:80,000-160,000

enviado en

dry ice

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.
IgG antibody is a glycoprotein, composed of two polypeptide chains (light and heavy), each possessing variable and constant domains. The variable region of IgG antibody is specific to antigens and is highly conserved.
Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for all mouse IgG subclasses. IgG is purified from mouse serum by fractionation and ion exchange chromatography. The purified IgG is then conjugated to peroxidase by protein cross-linking with 0.2% glutaraldehyde.

Especificidad

Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for all mouse IgG subclasses.

Inmunógeno

Purified mouse IgG

Aplicación

Epidermis protein extracts were generated from human mammary surgery specimens and subjected to western blot using HRP-conjugated rabbit anti-mouse IgG as the secondary at a 1:80000 dilution in TBSt/5% milk.
Anti-Mouse IgG (whole molecule)–Peroxidase antibody may be used in direct ELISA at a working antibody dilution of 1:40,000. For immunoblotting, a dilution of 1:80,000 to 1:160,000 may be used. A working antibody dilution of 1:200 is recommended for immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil or human appendix sections. Mouse IgG-peroxidase antibody was used at a dilution of 1:20 for immunohistochemistry of mice muscle tissue sections; a dilution of 1:500 was used for South-western blotting in the same study. For immunoblotting of human skeletal muscle tissue extracts, a dilution of 1:5000 was used.
Anti-Mouse IgG (whole molecule)–Peroxidase antibody has been used in western blotting , immunohistochemistry and protein pin array assay.

Acciones bioquímicas o fisiológicas

IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG antibody has its function similar to IgM antibody in complement system activation.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Nota de preparación

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Almacenamiento y estabilidad

Store at -20 °C for long term. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots -20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

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Certificado de origen

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Quotes and Ordering

Natural autoantibodies
Coutinho A, et al.
Current Opinion in Immunology, 7(6), 812-818 (1995)
Kristen J Nowak et al.
Annals of neurology, 61(2), 175-184 (2006-12-26)
To investigate seven congenital myopathy patients from six families: one French Gypsy, one Spanish Gypsy, four British Pakistanis, and one British Indian. Three patients required mechanical ventilation from birth, five died before 22 months, one is ventilator-dependent, but one, at
Rita Ferreira et al.
European journal of applied physiology, 97(3), 340-346 (2006-06-14)
The comprehension of the cellular mechanisms underlying skeletal muscle atrophy has been the aim of several experimental studies. However, the majority of them focused on alterations of the myocytes induced by different experimental conditions yet disregarding the contribution of other
mRNA and protein expression for novel GABA A receptors θ and ρ2 are altered in schizophrenia and mood disorders; relevance to FMRP-mGluR5 signaling pathway.
Fatemi SH, et al.
Translational Psychiatry, 3(6), e271-e271 (2013)
The Laboratory Rat (2000)

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