Merck
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A9418

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, BioReagent, suitable for cell culture

Sinónimos:
Albumin bovine serum, Bovine albumin, BSA
Número de CAS:
Número de EC:
Número MDL:
NACRES:
NA.75

origen biológico

bovine

línea de producto

BioReagent

formulario

lyophilized powder

mol peso

~66 kDa

technique(s)

cell culture | mammalian: suitable

pérdida

≤5%

pH

5.0-5.6

solubilidad

H2O: 40 mg/mL

enviado en

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids. It is a water-soluble protein with a molecular weight of 66.4kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

Aplicación

Bovine Serum Albumin has been used in the washing solution for flow cytometry analysis, tissue digestion solution and blocking solution for ELISA (enzyme-linked immunosorbent assay). It has also been used in the resuspension buffer for flow cytometry.

Envase

5, 10, 50, 100, 500 g in poly bottle

Acciones bioquímicas o fisiológicas

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Nota de preparación

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificado de Análisis

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Certificado de origen

Introduzca el número de lote para buscar un Certificado de origen (COO).

Hannah C Jeffery et al.
Journal of immunology (Baltimore, Md. : 1950), 203(5), 1151-1159 (2019-08-09)
There is no effective treatment for autoimmune biliary diseases. Therefore, understanding their immunopathology is crucial. The biliary epithelial cells (BEC), expressing TLR-4, are constantly exposed to gut microbes and bacterial wall LPS, and in settings of inflammation, the immune infiltrate
Intercellular adhesion molecule-1/LFA-1 cross talk is a proximate mediator capable of disrupting immune integration and tolerance mechanism at the feto-maternal interface in murine pregnancies.
Blois S et al.
Journal of Immunology, 174, 1820-1820 (2005)
Luisana Di Cristo et al.
International journal of environmental research and public health, 15(4) (2018-03-22)
Nanoparticles (NP)-based inhalation systems for drug delivery can be administered in liquid form, by nebulization or using pressurized metered dose inhalers, and in solid form by means of dry powder inhalers. However, NP delivery to the lungs has many challenges
Expression of ABCG2 and Bmi-1 in oral potentially malignant lesions and oral squamous cell carcinoma.
Dalley AJ et al.
Cancer Medicine, 3, 273-273 (2014)
Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and Frit-FAB LC/MS.
Hirayama K et al.
Biochemical and Biophysical Research Communications, 173, 639-646 (1990)

Artículos

Albumin in Cell Culture

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

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