Todas las fotos(3)

A9434

Sigma-Aldrich

Ammonium chloride

for molecular biology, suitable for cell culture, ≥99.5%

Sinónimos:
Salmiac
Fórmula lineal:
NH4Cl
Número de CAS:
Peso molecular:
53.49
Beilstein:
4371014
Número de EC:
Número MDL:
eCl@ss:
38050207
ID de la sustancia en PubChem:
NACRES:
NA.31

Nivel de calidad

200

grado

for molecular biology

densidad de vapor

1.9 (vs air)

presión de vapor

1 mmHg ( 160.4 °C)

ensayo

≥99.5%

formulario

powder or crystals

technique(s)

cell culture | mammalian: suitable

mp

340 °C (subl.) (lit.)

trazas de anión

phosphate (PO43-): ≤2 ppm
sulfate (SO42-): ≤20 ppm

trazas de catión

Fe: ≤2 ppm
heavy metals (as Pb): ≤5 ppm

actividad extraña

DNase, RNase, protease, none detected

SMILES string

N.Cl

InChI

1S/ClH.H3N/h1H;1H3

InChI key

NLXLAEXVIDQMFP-UHFFFAOYSA-N

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Categorías relacionadas

Aplicación

Ammonium chloride has been used:
  • as an inhibitor of endosome acidification in vero cells
  • as an autophagy inhibitor in vascular smooth muscle cell (VSMC)
  • in the depletion and lysis of erythrocytes in testis parenchyma homogenate

Envase

500 g in poly bottle
1 kg in poly bottle

Acciones bioquímicas o fisiológicas

Ammonium chloride is one of the three principal components of the nitrogen cycle. Ammonium chloride administration promotes creatinine and urea clearance andinduces metabolic acidosis in mice. Excess of ammonium chloride inhibits Kreb′s pathway in brain and depletes ATP in astrocytes. Ammonium chloride inhibits acidification in the endosome-lysosome system. Ammonium chloride is a hemolysing agent used for the lysis of erythrocytes.

pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Acute Tox. 4 Oral - Eye Irrit. 2

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

Certificado de Análisis

Certificado de origen

Infectious bronchitis virus entry mainly depends on clathrin mediated endocytosis and requires classical endosomal/lysosomal system
Wang H, et al.
Virology, 528, 118-136 (2019)
Aqueous ammonia and ammonium chloride hydrates: Principal infrared spectra
Max JJ and Chapados C
Journal of Molecular Structure, 1046, 124-135 (2013)
Nrf2 Activation Provides Atheroprotection in Diabetic Mice Through Concerted Upregulation of Antioxidant, Anti-inflammatory, and Autophagy Mechanisms
Lazaro I, et al.
Frontiers in Pharmacology, 9, 118-136 (2018)
Effect of ammonium chloride and dietary phosphorus in the azotaemic rat. I. Renal function and biochemical changes
Jara A, et al.
Nephrology, Dialysis, and Transplantation, 19(8), 1986-1992 (2004)
Inhibition of endosome-lysosome system acidification enhances porcine circovirus 2 infection of porcine epithelial cells
Misinzo G, et al.
Journal of Virology, 82(3), 1128-1135 (2008)

Protocolos

GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

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