This product is not specifically recommended for molecular biology. Per the Product Information Sheet located in the Documents section of this product page catalog numbers B6917, B8667, 126609, B8894, and B2518 are suitable for molecular biology applications.
A9647
Seroalbúmina bovina
heat shock fraction, pH 7, ≥98%
Sinónimos:
Albúmina bovina, Albúmina sérica bovina, BSA
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10 G
MXP 2,039.00
50 G
MXP 4,974.00
100 G
MXP 7,670.00
500 G
MXP 26,635.00
1 KG
MXP 43,988.00
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Servicio técnico
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Permítanos ayudarlebiological source
bovine
Quality Level
assay
≥98%
form
lyophilized powder
mol wt
~66 kDa
purified by
heat shock fractionation
origin
USA origin
technique(s)
immunocytochemistry: suitable
impurities
≤5.0% Loss on drying
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT virus, none detected
VSV virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
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Categorías relacionadas
1 of 4
Este artículo | A8022 | A7888 | A7906 |
|---|---|---|---|
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| technique(s) immunocytochemistry: suitable | technique(s) ELISA: suitable, blocking: suitable, electrophoresis: suitable, western blot: suitable | technique(s) blood typing: suitable | technique(s) blood typing: suitable, microbiological culture: suitable |
| assay ≥98% | assay ≥96% | assay ≥96% | assay ≥98% |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 300 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
General description
La seroalbúmina bovina es una proteína cuya principal función biológica es regular la presión osmótica coloidal de la sangre. La BSA se utiliza mucho en diversos estudios y aplicaciones. Compuesta por 583 aminoácidos,[1][2] la BSA es una proteína hidrosoluble con un peso molecular calculado de 66 430 Da. Seis hélices α forman los tres dominios homólogos de la BSA. Dependiendo del pH, experimenta isomerización conformacional reversible. La estructura nativa de la proteína se vuelve reactiva y flexible al calentarse.[3]
Application
La seroalbúmina bovina se utiliza para muchas aplicaciones, entre ellas las siguientes:
- La BSA se ha utilizado en cultivos celulares, por ejemplo, como suplemento en medios preparados para el cultivo de oligodendrocitos.[4]
- La BSA se ha utilizado para la dilución de las disoluciones de anticuerpo primario en los procedimientos inmunohistoquímicos.[5]
- La BSA se ha utilizado como patrón en el método Bradford de cuantificación de proteínas.[6]
- La BSA se ha utilizado en la disolución amortiguadora de bloqueo y permeabilización durante los estudios de inmunofluorescencia.[7]
Biochem/physiol Actions
Se sospecha que ciertos epítopos conformacionales y de secuencia primaria son alérgenos en las alergias humanas a la leche y a la carne.
Preparation Note
La seroalbúmina puede denominarse también Fracción V. Esta convención de nomenclatura procede del método original de fraccionamiento de las proteínas séricas de Cohn en el que se utiliza precipitación en etanol frío. Utilizando dicho método, la seroalbúmina se encontró en la fracción quinta del etanol. Desde entonces, algunos han venido utilizando la expresión «Fracción V» para describir la albúmina sérica, al margen del método de preparación. Otros han utilizado este término para describir la seroalbúmina purificada por métodos de fraccionamiento en etanol que se han modificado mucho desde que se describió el método Cohn original. Sigma-Aldrich fabrica y distribuye seroalbúminas purificadas mediante una variedad de métodos primarios, entre ellos el verdadero método de fraccionamiento de Cohn, los métodos de fraccionamiento en etanol modificados, el fraccionamiento térmico y la cromatografía. Etapas de purificación añadidas pueden ser la cristalización o la filtración en carbón activado.
Se prepara utilizando fraccionamiento térmico
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Referencia del producto
Descripción
Precios
Clase de almacenamiento
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Mohammad Bahrami et al.
Scientific reports, 9(1), 11739-11739 (2019-08-15)
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Artículos
This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.
Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.
Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.
Protocolos
To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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Can A9647 be used in polymerase reactions?
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What buffer the buffer it is lyophilized in?
1 answer-
The albumin is heat shock fractionated from bovine serum. No buffer is added during this process.
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Is this product protease-free?
1 answer-
This product has not been tested for protease activity. Please see the link below to review other BSA products that are listed as protease-free:
https://www.sigmaaldrich.com/product/compare?term=&focus=products&page=1&perpage=30&sort=relevance&type=product&products=A7030%3ASIGMA%3Asial%2CA3059%3ASIGMA%3Asial%2CA3294%3ASIGMA%3AsialHelpful?
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Is this product suitable for using as protein standard when measuring concentration by spectrophotometry?
1 answer-
This product has been cited for as use protein standard in Bradford's method for spectrophotometric determination of protein. The article is accessible at this link: https://www.sciencedirect.com/science/article/pii/S0946672X20302698
Please refer to the table for a description of each albumin product. Based on customer input and literature reports, the following table lists product numbers which have successfully been used for specific applications. The list is not comprehensive, and product numbers not listed may often be substituted. It is available at this link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/111/538/albumin-application-selection-guide.pdf
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What is the difference between Albumin A7906 and Albumin A9647 ?
1 answer-
Product A9647 and A7906 are identical. The only difference is that product A7906 offers 5 KG and 10 KG pack sizes.
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How can the concentration of a solution of Product A9647, Albumin from bovine serum, be quantified?
1 answer-
The concentration of a solution of BSA can be measured by using the extinction coefficient (E1%) of 6.67. The 1% refers to a solution that is 1% weight-to-volume (i.e., 10 mg/mL).
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What is the molecular weight of Product A9647, Albumin from bovine serum?
1 answer-
The molecular weight of BSA is 66,430.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the pH of a solution of Product A9647, Albumin from bovine serum?
1 answer-
The pH of a 1% solution is approximately 7.0, according to the product specifications.
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