If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
B4639
Biotina
powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sinónimos:
D-Biotina, Bios II, Coenzima R, Vitamina H, Vitaminas B7
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MXP 1,021.00
MXP 2,661.00
MXP 4,778.00
MXP 16,881.00
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Nombre del producto
Biotina, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
InChI key
YBJHBAHKTGYVGT-ZKWXMUAHSA-N
InChI
1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1
SMILES string
[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
product line
BioReagent
assay
≥99%
form
powder
mol wt
Mw 244.31 g/mol
technique(s)
cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable
color
white to off-white
mp
231-233 °C (lit.)
solubility
H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.)
storage temp.
2-8°C
Quality Level
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Categorías relacionadas
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Este artículo | B4501 | 91827 | PHR1233 |
|---|---|---|---|
| technique(s) cell culture | insect: suitable, cell culture | plant: suitable, cell culture | mammalian: suitable | technique(s) HPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, gas chromatography (GC): suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| assay ≥99% | assay ≥99% (HPLC) | assay - | assay - |
| form powder | form lyophilized powder | form - | form - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-30°C |
| solubility H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.) | solubility ammonium hydroxide: 50 mg/mL (2 M) | solubility - | solubility - |
| mp 231-233 °C (lit.) | mp 231-233 °C (lit.) | mp 231-233 °C (lit.) | mp 231-233 °C (lit.) |
Application
- como un suplemento de los medios M199 en sistemas de cultivo in vitro[1]
- como un componente del medio exento de suero SATO para el aislamiento de los precursores de los oligodendrocitos corticales (OPC)[2]
- en cultivo con precursores bioquímicos para la síntesis de novo de ácidos grasos[3]
- como un componente en los medios de cultivo agregados[4]
Biochem/physiol Actions
General description
Related product
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Artículos
Importance and uses of biotin in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Contenido relacionado
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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If I would like to make 1M biotin stock (B4639), what would be the best way?
1 answer-
A 1 M solution of biotin would require a concentration of 244 mg/mL. The maximum solubility tested for this compound is 50mg/ml in 2M NaOH or NH4OH, which results in a 204 mM solution. Similar rates have been reported for DMSO. Heat and agitation may improve the solubility. However, a precipitate may form upon cooling. A search of the literature did not reveal a more efficient solvent for Biotin. The preparation of a 1M solution would have to be determined experimentally.
Helpful?
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Hello, How can I reconstruct biotin powder (B4639)? which solvent? and how can I prepare 50mM biotin solution for 1 ml final solution?
1 answer-
This product is soluble in 50 mg/ml 2 M NH4OH.
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