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C3260

Sigma-Aldrich

Citrate Synthase from porcine heart

ammonium sulfate suspension, ≥100 units/mg protein

Synonym(s):
Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)
CAS Number:
Enzyme Commission number:
Número MDL:
NACRES:
NA.54

origen biológico

Porcine heart

Nivel de calidad

200

formulario

ammonium sulfate suspension

specific activity

≥100 units/mg protein

mol peso

85 kDa

solubilidad

H2O: soluble 1.0 mg/mL, clear

actividad extraña

isocitrate dehydrogenase and aconitase ≤0.01%
malic dehydrogenase ≤0.1%

temp. de almacenamiento

2-8°C

Aplicación

Citrate Synthase from porcine heart has been used:
  • to inject newt egg for determining its importance in egg activation at fertilization[1]
  • in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity[2]
  • to examine whether it can induce a [Ca2+] increase and egg activation in unfertilized eggs[3]

Acciones bioquímicas o fisiológicas

Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.

Definición de unidad

One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.

Forma física

Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0.

Nota de preparación

Dissolves in water to form a clear solution at 1 mg/mL concentration.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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