C3260

Citrate Synthase from porcine heart

ammonium sulfate suspension, ≥100 units/mg protein

• Synonym(s): Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)
• CAS Number: 9027-96-7
• Enzyme Commission number: 4.1.3.7 (BRENDA, IUBMB)
• Número MDL: MFCD00130808
• NACRES: NA.54

Properties

• origen biológico: Porcine heart
• Nivel de calidad: 200
• formulario: ammonium sulfate suspension
• specific activity: ≥100 units/mg protein
• mol peso: 85 kDa
• solubilidad: H₂O: soluble 1.0 mg/mL, clear
• actividad extraña: isocitrate dehydrogenase and aconitase ≤0.01%; malic dehydrogenase ≤0.1%
• temp. de almacenamiento: 2-8°C

Description

Aplicación

Citrate Synthase from porcine heart has been used:

• to inject newt egg for determining its importance in egg activation at fertilization[1]
• in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity[2]
• to examine whether it can induce a [Ca²⁺] increase and egg activation in unfertilized eggs[3]

Acciones bioquímicas o fisiológicas

Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H₂O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.

Definición de unidad

One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.

Forma física

Suspension in 3.2 M (NH₄)₂SO₄ solution, pH 7.0.

Nota de preparación

Dissolves in water to form a clear solution at 1 mg/mL concentration.

Safety Information

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable