Merck
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C4129

Sigma-Aldrich

α-Chymotrypsin from bovine pancreas

Type II, lyophilized powder, ≥40 units/mg protein

Synonym(s):
α-chymotrypsin A and B, alpha-chymotrypsin
CAS Number:
Enzyme Commission number:
Número de EC:
Número MDL:
eCl@ss:
42010112

tpo

Type II

Nivel de calidad

300

formulario

lyophilized powder

specific activity

≥40 units/mg protein

mol peso

25 kDa

composición

protein, ≥85%

color

white to off-white

Nº de acceso UniProt

application(s)

diagnostic assay manufacturing

temp. de almacenamiento

−20°C

Gene Information

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Aplicación

The enzyme from Sigma has been used to study the structure-function relationship in glycosylated α-chymotrypsin using immobilized metal-ion affinity chromatography (IMAC) and immobilized metal-ion affinity capillary electrophoresis (IMACE).[1]

Envase

250, 500 mg in poly bottle
1, 10 g in poly bottle

Acciones bioquímicas o fisiológicas

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.
A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Definición de unidad

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Nota de preparación

Produced from 3× crystallized chymotrypsinogen

Nota de análisis

Protein determined by E1%/280

Otras notas

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Technical Information

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