Merck

C5585

Sigma-Aldrich

Monoclonal Anti-Biotin–Cy3 antibody produced in mouse

clone BN-34, purified immunoglobulin, buffered aqueous solution

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Sinónimos:
Monoclonal Anti-Biotin
NACRES:
NA.46

origen biológico

mouse

Nivel de calidad

conjugado

CY3 conjugate

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

BN-34, monoclonal

formulario

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
indirect immunofluorescence: 1:200

isotipo

IgG1

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

target post-translational modification

unmodified

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1 of 4

Este artículo
C3117A1559A0185
conjugate

CY3 conjugate

conjugate

biotin conjugate

conjugate

agarose conjugate

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

BN-34, monoclonal

clone

CY-96, monoclonal

clone

BN-34, monoclonal

clone

BN-34, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

PBS suspension

form

-

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400, indirect immunofluorescence: 1:200

technique(s)

direct ELISA: suitable, dot blot: suitable, immunocytochemistry: 1-2 μg/mL using chicken fibroblasts, microarray: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

dot blot: suitable, flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, indirect ELISA: 1:30,000, western blot (chemiluminescent): 1:80,000

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Descripción general

Monoclonal Anti-Biotin (mouse IgG1 isotype) is derived from the BN-34 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Biotin is a naturally occurring vitamin and co-enzyme required by cells in living organisms or in culture.
The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

Inmunógeno

Biotin-KLH conjugate

Aplicación

Monoclonal Anti-Biotin−Cy3 antibody produced in mouse has been used in:
  • fluorescent microscopy
  • RNA fluorescence in situ hybridization (FISH){36
  • immunostaining

The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Acciones bioquímicas o fisiológicas

Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.
Monoclonal Anti-Biotin- Cy3 can be used in many other applications where biotin can be introduced as target label. It can be used in detection of DNA and mRNA by sensitive non-isotopic in situ hybridization. It may also be used for detection of microinjected biotin-haptenized cytoskeletal proteins allowing examination of the pattern of incorporation and turnover of cytoskeletal proteins in living cells.

Forma física

Solution in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Información legal

Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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