Anti-Actin, α-Smooth Muscle - Cy3 antibody, Mouse monoclonal

clone 1A4, purified from hybridoma cell culture

Monoclonal Anti-Actin, α-Smooth Muscle, SMA

Quality Level

biological source


antibody form

purified immunoglobulin

antibody product type

primary antibodies


1A4, monoclonal


buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

human, frog, sheep, chicken, goat, bovine, rat, guinea pig, mouse, canine, rabbit, snake

storage condition

protect from light


immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tonsil or appendix




CY3 conjugate

Featured Industry

Research Pathology

UniProt accession no.

shipped in

wet ice

storage temp.


Gene Information

human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

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General description

Monoclonal Anti-Actin, α-Smooth Muscle (mouse IgG2a isotype) is derived from the 1A4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice.
The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.


N-terminal synthetic decapeptide of α-smooth muscle actin.


Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (2 papers)
Immunocytochemistry (3 papers)
Immunofluorescence (13 papers)
Immunohistochemistry (27 papers)
Western Blotting (2 papers)
Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.

Biochem/physiol Actions

Smooth muscle actin is transiently coexpressed with sarcomeric α-actin during myogenesis in chicken and rat embryos. It has been found in the ventricular conducting tract of adult mammalian heart. It is expressed in leiomyomas, leiomyosarcomas and leiomyoblastomas, as well as in a proportion of rhabdomyosarcomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide, as a preservative.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


12 - Non Combustible Liquids

WGK Germany


Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis

Certificado de origen

Coexpression of alpha-sarcomeric actin, alpha-smooth muscle actin and desmin during myogenesis in rat and mouse embryos I. Skeletal muscle
Babai F, et al.
Differentiation, 44(2), 132-142 (1990)
Debby Gawlitta et al.
Tissue engineering. Part A, 18(1-2), 208-218 (2011-08-24)
To ensure the survival of engineered bone after implantation, we combined human endothelial colony forming cells (ECFCs) and multipotent stromal cells (MSCs) as a proof of concept in a co-culture model to create in vitro prevascularized bone constructs. We hypothesized...
Jinhua Li et al.
The American journal of pathology, 175(4), 1380-1388 (2009-09-05)
Diabetic nephropathy is the leading cause of chronic renal failure. Myofibroblasts play a major role in the synthesis and secretion of extracellular matrix in diabetic renal fibrosis. Increasing evidence suggests that endothelial cells may undergo endothelial-myofibroblast transition under physiological and...
C Konoeda et al.
Transplantation proceedings, 45(5), 1797-1801 (2013-06-19)
Aberrant epithelial repair is a crucial event in the airway remodeling that characterizes obliterative bronchiolitis (OB) in transplanted lungs. Recent data from experiments using epithelial cell lines and human airway tissues from lung transplant recipients suggest that epithelial to mesenchymal...
Patricia Parsons-Wingerter et al.
The American journal of pathology, 167(1), 193-211 (2005-06-24)
Integrin alpha5beta1 is among the proteins overexpressed on tumor vessels and is a potential target for diagnostics and therapeutics. Here, we mapped the distribution of alpha5beta1 integrin in three murine tumor models and identified sites of expression that are rapidly...

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