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C9999

Sigma-Aldrich

Citrate Buffer, pH 6.0, 10×, Antigen Retriever

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NACRES:
NA.47

formulario

liquid

Nivel de calidad

100

pH

6.0

application(s)

hematology
histology

temp. de almacenamiento

2-8°C

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Este artículo
E1161T6455R2283
Sigma-Aldrich

Sigma-Aldrich

C9999

Citrate Buffer, pH 6.0, 10×, Antigen Retriever

Sigma-Aldrich

Sigma-Aldrich

E1161

EDTA Buffer, pH 8.5, 10×, Antigen Retriever

Sigma-Aldrich

Sigma-Aldrich

T6455

Tris-HCl Buffer, pH 10, 10×, Antigen Retriever

Sigma-Aldrich

Sigma-Aldrich

R2283

Pepsin Reagent, Ready to Use, Antigen Retriever

pH

6.0

pH

8.5

pH

10

pH

-

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

hematology
histology

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

Aplicación

Citrate Buffer, pH 6.0, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies or DNA fluorescent in situ hybridization. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of Citrate buffer, pH 6.0, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens.

Nota de preparación

Dilute the Citrate buffer, pH 6.0, 10x, Antigen Retriever 10-fold with water to prepare a 1x Working Solution, e.g., dilute 10 mL of 10x concentrate with 90 mL of water.

Almacenamiento y estabilidad

Store the product at 2–8 °C. Do Not Freeze to prevent possible precipitation.

Otras notas

Occasionally the buffer may contain a yellowish tinge. This is due to preservative and will not affect the function of the buffer.
Store the prepared 1′ Citrate buffer, pH 6.0, Working Solution at 2–8 °C.

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Referencia del producto
Descripción
Precios

pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H317 - H412

Clasificaciones de peligro

Aquatic Chronic 3 - Skin Sens. 1

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

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T1503
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25G
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