COIPP

Immunoprecipitation Vector Kit

• NACRES: NA.85

Properties

• grado: for molecular biology
• Nivel de calidad: 200
• formulario: buffered aqueous solution
• enviado en: dry ice
• temp. de almacenamiento: −20°C

Description

Descripción general

The Immunoprecipitation (IP) Vector Kit provides a unique method to validate protein-protein interactions in a convenient 96-well format. This system enables potential binding partners for the protein of interest to be tagged with ”FLAG and c-Myc epitopes, followed by capture and detection using the ANTI-FLAG M2 High Sensitivity Capture Plate.

The kit provides expression vectors ”pFLAG-CMV”-2 and pc-Myc-CMV-2 designed for the cloning and expression of protein interaction candidates as N-terminal FLAG and N-terminal c-Myc fusions. Control plasmids pFLAG-CMV-2-p53, pC-Myc-CMV-2-Large T antigen and pC-Myc-CMV-2-BAP supplied are for expression of positive and negative binding partners in the IP analysis. The kit also includes the MF-2 and MR-2 verification primers.

Vector Maps and Sequences

Envase

1 kit

Características y beneficios

• Fast and convenient 96 well plate format
• Cleaner analysis due to strongly bound antibody
• Rapid, sensitive, quantitative results

Componentes

The IP Vector Kit contains:

• pFLAG-CMV-2 Expression Vector (10 μg)
• pc-Myc-CMV-2 Expression Vector (10 μg)
• pFLAG-CMV-2-p53 control plasmid (20 μg)
• pc-Myc-CMV-2-Large T antigen control plasmid (10 μg)
• pc-Myc-CMV-2-BAP control plasmid (10 μg)
• Verification Primer-MF (2 nmol)
• Verification Primer-MR (2 nmol)

Kit Components Only

• pc-Myc-CMV-2 10 μg
• pc-Myc-CMV-2-BAP 10 μg
• pc-Myc-CMV-2-Large T antigen 10 μg
• pFLAG-CMV™-2 10 μg
• pFLAG-CMV™-2-p53 20 μg
• Verification Primer-MF 2 nmol
• Verification Primer-MR 2 nmol

Safety Information

• Código de clase de almacenamiento: 13 - Non Combustible Solids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable