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Merck

D2650

Sigma-Aldrich

Dimetilsulfóxido

Dimethyl sulfoxide

Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Sinónimos:

DMSO

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100 ML
MXP 6,011.00

MXP 6,011.00


Fecha estimada de envío05 de marzo de 2025



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100 ML
MXP 6,011.00

About This Item

Fórmula lineal:
(CH3)2SO
Número de CAS:
Peso molecular:
78.13
Beilstein/REAXYS Number:
506008
EC Number:
MDL number:
UNSPSC Code:
12191502
PubChem Substance ID:
NACRES:
NA.75
grade:
Hybri-Max
assay:
≥99.7%
technique(s):
cell culture | hybridoma: suitable
cryopreservation: suitable
bp:
189 °C (lit.)
vapor pressure:
0.42 mmHg ( 20 °C)

MXP 6,011.00


Fecha estimada de envío05 de marzo de 2025


grade

Hybri-Max

Quality Level

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

sterility

sterile-filtered

product line

BioReagent

assay

≥99.7%

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

technique(s)

cell culture | hybridoma: suitable
cryopreservation: suitable

impurities

endotoxin, tested
≤0.1% water
water

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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Application

El DMSO es un disolvente aprótico polar utilizado en reacciones químicas, en reacciones en cadena de la polimerasa (PCR) y como agente de vitrificación crioprotector para la conservación de células, tejidos y órganos. El DMSO se utiliza en los medios de congelación celular para proteger a las células de las lesiones mecánicas inducidas por el cristal de hielo. Se utiliza para el almacenamiento en congelación de líneas celulares de hibridomas y heteroploides primarias, subcultivadas y recombinantes; células madre embrionarias (ESC) y células madre hematopoyéticas. El DMSO se utiliza con frecuencia en combinación con la seroalbúmina bovina (BSA) o el suero bovino fetal (FBS).

Packaging

100 ml en botella de vidrio
El embalaje de ampollas de vidrio está ahora disponible bajo un nuevo número de referencia, D2653

Other Notes

Este producto es un producto Hybri-Max. Se analiza en hibridomas y se evalúa su idoneidad para la congelación celular. Este producto está esterilizado mediante filtración (0,2 micras) y probado para determinar la concentración de endotoxinas.

Caution

Fácilmente superenfriado, vuelve a fundir lentamente a temperatura ambiente. Producto solidificado que puede volver a licuarse por calentamiento a temperatura ambiente sin detrimento del producto.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Certificados de análisis (COA)

Lot/Batch Number

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Protocolos

Human ips cell passaging protocol using EZ-LiFT™ Reagent, an enzyme-free and chemically defined pluripotent stem cell dissociation reagent.

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

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Questions

1–10 of 17 Questions  
  1. Is it safe in terms of health and product safety to store a leftover DMSO ampule covered with parafilm in a flammable cabinet?

    1 answer
    1. DMSO is hygroscopic and light-sensitive. The storage container must protect from light and moisture uptake. Since this DMSO is sterile-filtered and intended for cell culture applications, the storage containers must also be sterile and endotoxin-free. It is recommended to purge aliquots and store them under inert gas, in a compatible, tightly sealed container, at a cool storage area, such as 4°C.

      DMSO is not compatible with many types of plastics, so it is advisable to store it in appropriate amber glass vials for long-term storage. Please refer to the Product Information Sheet and the Safety Data Sheet at the link below for compatibility of DMSO with various materials and for further details:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265

      Helpful?

  2. Is this anhydrous?

    1 answer
    1. This product is not considered anhydrous. The maximum allowable water content is 0.1% which is reported on the lot-specific Certificate of Analysis. Please see the link below to review the anhydrous DMSO products available:
      https://www.sigmaaldrich.com/search/dmso-anhydrous?focus=products&page=1&perpage=30&sort=relevance&term=DMSO%20anhydrous&type=product

      Helpful?

  3. I ordered 10 ml glass vials, used 7 ml, Can I store it in centrifuge tubes? For how long can I use that?

    1 answer
    1. It may be best to store DMSO in amber glass vials for long term storage, as this compound is not compatible with many types of plastics. The compatibility of DMSO with various materials is listed below:
      Compatible: LDPE, HDPE, polypropylene, PPCO(polypropylene copolymer), polymethylpentene, nylon, teflon FEP
      Moderately compatible: polystyrene, ECTFE/ETFE
      Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonate

      Note that DMSO is hygroscopic and light sensitive. The storage container selected must offer protection from light and moisture uptake. The product is assigned a firm expiration date. Please refer to the lot specific Certificate of Analysis to assume the material is within the expected shelf life.

      Helpful?

  4. E' solo ad uso per ricerca o utilizzabile anche in diagnostica/human? Grazie

    1 answer
    1. This product is not suitable for diagnostic/human use.

      Helpful?

  5. Is this product packaged in a bottle with a septa cap?

    1 answer
    1. No, this product is not packaged with a septa cap. It comes in a 125 ml amber glass bottle with a 28 mm solid white cap.

      Helpful?

  6. Can I use it for PCR?

    1 answer
    1. While this product is tested specifically for use in cell culture, there is no reason to suspect that it would not perform well in PCR applications. The product is NOT tested for amplification, DNAse, or nuclease activity. Should this testing be important, see the link below to product options that include this testing:
      D9170 DMSO PCR Reagent:
      https://www.sigmaaldrich.com/product/sigma/d9170
      D8418 DMSO Molecular Biology Tested
      https://www.sigmaaldrich.com/US/en/product/sigma/d8418

      Helpful?

  7. what is the recommended re-test date & for how long can it be used beyond the expiry date mentioned in the COA?

    1 answer
    1. This product is assigned a firm expiration date of approximately 2 years. It is not assigned a retest date. The stability beyond the lot-specific expiration date is not determined and the quality of the product cannot be guaranteed. See the link below to review a sample Certificate of Analysis: https://www.sigmaaldrich.com/coa/SIGMA/D2650/RNBK5742.

      Helpful?

  8. Does DMSO have any synthetic utility?

    1 answer
    1. DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

      Helpful?

  9. What is the molarity of DMSO?

    1 answer
    1. DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

      Helpful?

  10. What type of filter is used to sterile-filter Dimethyl sulfoxide (DMSO)?

    1 answer
    1. DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.

      Helpful?

1–10 of 17 Questions  

Reviews

Active Filters

  1. Turkey
    • Review 1
    • Votes 3
    5 out of 5 stars.

    Is this product already concentration adjusted

    Is this product already concentration adjusted, as you mentioned this product used %8.7 concentration bu the thing i cannot understand that is this item already diluted for ready to use or do we have to dilute it what concentration we need ?

    Helpful?

  2. 9 Ratings-Only Reviews

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