Merck

D4941

Sigma-Aldrich

Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal

clone DKPS308, purified from hybridoma cell culture

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Sinónimos:
Anti-DAPK, Anti-ROCO3
Número MDL:
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

purified from hybridoma cell culture

antibody product type

primary antibodies

clon

DKPS308, monoclonal

formulario

buffered aqueous solution

mol peso

antigen ~160 kDa

species reactivity

human

technique(s)

immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using 293T (human embryonal kidney) cells transfected with DAP-kinase expression vector.

isotipo

IgG1

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

target post-translational modification

phosphorylation (pSer308)

Gene Information

human ... DAPK1(1612)

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Este artículo
D1319D2178P2859
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

ascites fluid

antibody form

purified immunoglobulin

clone

DKPS308, monoclonal

clone

polyclonal

clone

DAPK-55, monoclonal

clone

NM11, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

buffered aqueous solution

mol wt

antigen ~160 kDa

mol wt

antigen ~160 kDa

mol wt

antigen 160 kDa

mol wt

antigen 300 kDa

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Descripción general

Monoclonal Anti-phospho DAP-Kinase (pSer308) (mouse IgG1 isotype) is derived from the DKPS308 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a phosphopeptide of human DAP-kinase, conjugated to KLH. Death Associated Protein Kinase (DAPK) is characterized with a multidomain structure including subdomain typical of serine/threonine kinases, a Ca2+/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. In addition, it also contains two auto-inhibitory domains, one of them Ca2+/calmodulin dependent. In the absence of this latter domain, DAPK is constitutively active.

Inmunógeno

Phosphopeptide corresponding to amino acids 303-312 (pSer308) of human DAP-kinase, conjugated to KLH.

Aplicación

Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal has been used in:
  • western blot assay
  • immunocytochemistry
  • enzyme linked immunosorbent assay (ELISA)
  • immunoprecipitation

Monoclonal anti-phospho-DAP-kinase (pSer308) antibody can be used in indirect ELISA, immunoblotting and immunoprecipitation. It can also be used in western blotting.

Acciones bioquímicas o fisiológicas

Death Associated Protein Kinase (DAPK) plays crucial role in programmed cell death as well as in autophagy. DAPK activity is regulated by phosphorylation. Autophosphorylation at Ser308 on the calmodulin regulatory domain negatively regulate DAPK activity. This autophosphorylation, which occurs in cells at the basal state, lowers the affinity of DAPK for calmodulin and thus the kinase is inactive. Under some apoptotic conditions DAPK undergoes dephosphorylation. Consequently, it binds to calmodulin with higher affinity, becomes activated, phosphorylates its downstream substrate proteins, and mediates apoptosis. Monoclonal anti-phospho-DAP-kinase (pSer308) antibody can be used to study the mechanism of DAPK activation in apoptosis. It can also be used in microarray.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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The Pro-apoptotic Function of Death-associated Protein Kinase Is Controlled by a Unique Inhibitory Autophosphorylation-based Mechanism
Shohat G, et al.
Test, 276, 47460-47467 (2001)
Song Liu et al.
Frontiers in neuroscience, 15, 525615-525615 (2021-03-12)
Excitatory toxicity due to excessive glutamate release is considered the core pathophysiological mechanism of cerebral ischemia. It is primarily mediated by N-methyl-D-aspartate receptors (NMDARs) on neuronal membranes. Our previous studies have found that icaritin (ICT) exhibits neuroprotective effects against cerebral
A functional genetic screen identifies regions at the C-terminal tail and death-domain of death-associated protein kinase that are critical for its proapoptotic activity
Raveh T, et al.
Proceedings of the National Academy of Sciences of the USA, 97, 1572-1577 (2000)
The role of DAPK-BimEL pathway in neuronal death induced by oxygen-glucose deprivation
He C, et al.
Neuroscience, 258, 254-254 (2014)
Xiao-Hui Li et al.
Neural regeneration research, 18(8), 1795-1801 (2023-02-09)
We previously showed that death-associated protein kinase 1 (DAPK1) expression is increased in hippocampal tissue in a mouse model of major depressive disorde and is related to cognitive dysfunction in Alzheimer's disease. In addition, depression is a risk factor for

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