Merck
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D5671

SAFC

Dulbecco′s Modified Eagle′s Medium - high glucose

With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonym(s):
DME, DMEM
NACRES:
NA.75

Nivel de calidad

400

esterilidad

sterile-filtered

formulario

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurezas

endotoxin, tested

componentes

glucose: high
NaHCO3: yes
sodium pyruvate: no
L-glutamine: no
phenol red: yes
HEPES: no

enviado en

ambient

temp. de almacenamiento

2-8°C

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Descripción general

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Aplicación

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Reconstitución

Supplement with 0.584 g/L L-glutamine.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

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