Merck
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D7876

Sigma-Aldrich

Diamine Oxidase from porcine kidney

≥0.05 unit/mg solid

Synonym(s):
Amine:oxygen oxidoreductase (deaminating) (pyridoxal-containing)
CAS Number:
Enzyme Commission number:
Número de EC:
Número MDL:
NACRES:
NA.54

origen biológico

Porcine kidney

Nivel de calidad

200

formulario

solid

specific activity

≥0.05 unit/mg solid

mol peso

170 kDa

solubilidad

100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL

actividad extraña

monoamine oxidase (benzylamine substrate) ≤1%

temp. de almacenamiento

−20°C

Descripción general

Diamine oxidase from porcine kidney is a homodimer consisting of two equal subunits with a molecular weight of 87 kDa each. Each subunit contains one molecule of pyridoxal phosphate and one atom of copper. The molecular mass of the enzyme is found to be 170 kDa. The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% N-acetylglucosamine, and 0.25% N-acetylneuraminic acid. The enzyme exhibits a high affinity for concanavalin A. Optimum pH with cadverine and histamine as substrates is found to be 6.3-7.4.

Aplicación

Diamine Oxidase from porcine kidney has been used in the construction of histamine biosensor.
Diamine oxidase from porcine kidney has been used in a study to investigate a luminescence-based test for determining ornithine decarboxylase activity. Diamine oxidase from porcine kidney has also been used in a study to investigate N-linked oligosaccharide structures in diamine oxidase.

Envase

250 mg in poly bottle
1, 5, 10 g in poly bottle

Acciones bioquímicas o fisiológicas

Diamine Oxidase catalyzes the oxidation of monoamines, diamines, and histamine to aldehydes, ammonia, and hydrogen peroxide. The enzyme is classified as a copper amine oxidase and it is a key enzyme in nitrogen metabolism. Diamine oxidase is inhibited by diethyldithiocarbamate, phenylhydrazine, semicarbazide, cyanide, isonicotinic acid hydrazide.

Definición de unidad

One unit will oxidize 1.0 μmole of putrescine per hr at pH 7.2 at 37 °C.

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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