Merck
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D8130

Sigma-Aldrich

5,5′-Dithiobis(2-nitrobenzoic acid)

greener alternative

≥98%, BioReagent, suitable for determination of sulfhydryl groups

Sinónimos:
DTNB, Bis(3-carboxy-4-nitrophenyl) disulfide, Ellman’s Reagent, 3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid
Fórmula lineal:
[-SC6H3(NO2)CO2H]2
Número de CAS:
Peso molecular:
396.35
Beilstein:
1896821
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.26

línea de producto

BioReagent

ensayo

≥98%

formulario

powder

características de los productos alternativos más sostenibles

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Use of Renewable Feedstocks
Learn more about the Principles of Green Chemistry.

mp

240-245 °C (dec.) (lit.)

idoneidad

suitable for determination of sulfhydryl groups

categoría alternativa más sostenible

Re-engineered

SMILES string

OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

InChI key

KIUMMUBSPKGMOY-UHFFFAOYSA-N

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Descripción general

5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.

Aplicación

Ellman′s reagent maybe used for acetylcholinesterase assay, and to specifically label cysteine residues. It is a sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues.

Envase

500 mg in glass insert
1, 5, 10, 25, 100 g in poly bottle

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

Certificado de Análisis

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Certificado de origen

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Christian K Riener et al.
Analytical and bioanalytical chemistry, 373(4-5), 266-276 (2002-07-12)
Since its introduction in 1959, Ellman's reagent (5,5'-dithio-bis(2-nitrobenzoic acid)) has been the favorite reagent for spectrophotometric measurement of protein sulfhydryls. Meanwhile however, evidence has accumulated that many protein sulfhydryls give an incomplete reaction with Ellman's reagent, even during prolonged assay
Reassessment of Ellman's reagent.
P W Riddles et al.
Methods in enzymology, 91, 49-60 (1983-01-01)
null
Ellman's reagent: 5,5'-dithiobis(2-nitrobenzoic acid)--a reexamination.
P W Riddles et al.
Analytical biochemistry, 94(1), 75-81 (1979-04-01)
María Elena Quintanilla et al.
Frontiers in behavioral neuroscience, 14, 122-122 (2020-08-28)
Chronic ethanol intake results in brain oxidative stress and neuroinflammation, which have been postulated to perpetuate alcohol intake and to induce alcohol relapse. The present study assessed the mechanisms involved in the inhibition of: (i) oxidative stress; (ii) neuroinflammation; and

Protocolos

Enzymatic Assay of Chloramphenicol Acetyltransferase

This procedure applies to products that have a specification for the enzymatic activity of chloramphenicol acetyltransferase.

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