Merck
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D8418

Sigma-Aldrich

Dimethyl sulfoxide

for molecular biology

Synonym(s):
Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO
Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein:
506008
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.52

grado

for molecular biology

Nivel de calidad

densidad de vapor

2.7 (vs air)

presión de vapor

0.42 mmHg ( 20 °C)

Línea del producto

BioReagent

ensayo

≥99.9%

formulario

liquid

temp. de autoignición

573 °F

caducidad

Recommended retest period - 2 years

lim. expl.

42 %, 63 °F

technique(s)

DNA sequencing: suitable
PCR: suitable
transfection: suitable

impurezas

≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)

color

colorless

índice de refracción

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubilidad

H2O: miscible (completely)

densidad

1.10 g/mL (lit.)

absorción

passes test

idoneidad

suitable for molecular biology

actividad extraña

DNase and RNase, none detected

temp. de almacenamiento

room temp

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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Descripción general

Features and Benefits:

  • Suitable for Molecular Applications
  • Nuclease-Free DMSO
  • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
  • Molecular Biology Grade DMSO

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Aplicación

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in

  • polymerase chain reaction (PCR)
  • amplification of cDNA libraries
  • DNA sequencing
  • column-loading buffers for poly(A)+ RNA selection
  • buffers for the transformation of competent E. coli
  • transfection protocols.

Dimethyl sulfoxide has been used-

  • as an oligonucleotide solvent in array spotting[1]
  • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
  • for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
  • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
  • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.

Envase

1 vial
1, 1.5, 5.5, 7.5, 50, 50, 100, 100, 250, 250, 500 mL in glass bottle
1 L in glass bottle

Características y beneficios

This product is free from DNases, RNases, phosphatases and proteases.

Precaución

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 1

Punto de inflamabilidad F

188.6 °F - closed cup

Punto de inflamabilidad C

87 °C - closed cup

Equipo de protección personal

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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