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E5134

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)

Sinónimos:
Edetate disodium salt dihydrate, Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, Edathamil, EDTA-Na2, Sequestrene Na2
Empirical Formula (Hill Notation):
C10H14N2Na2O8 · 2H2O
Número de CAS:
Peso molecular:
372.24
Beilstein:
3900609
EC Number:
PubChem Substance ID:
NACRES:
NA.31

Quality Level

grade

for molecular biology

assay

99.0-101.0% (titration)

form

powder

application(s)

electrophoresis: suitable

impurities

≤0.005% Insolubles
≤0.1% Nitrilotriacetic acid (NTA)

mp

248 °C (dec.) (lit.)

solubility

3  M NaOH: 1.6 g/10 mL

cation traces

Fe: ≤0.005%
Pb: ≤0.002%

foreign activity

DNase, RNase, NICKase and protease, none detected

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

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General description

Ethylenediaminetetraacetic acid disodium salt dehydrate is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Application

Ethylenediaminetetraacetic acid disodium salt dehydrate has been used:
  • To prepare TE buffer.
  • As an anticoagulant.
  • As a constituent of Flow buffer.
Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Packaging

50, 100, 250, 500 g in poly bottle
1 kg in poly bottle
5 kg in poly drum

pictograms

Exclamation markHealth hazard

signalword

Warning

Hazard Classifications

Acute Tox. 4 Inhalation - Aquatic Chronic 3 - STOT RE 2 Inhalation

Target Organs

Respiratory Tract

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 2

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificado de Análisis

Certificado de origen

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can solutions of Product E5134, EDTA disodium salt, be autoclaved?

    Yes.

  4. Is Product E5134, EDTA disodium salt, stable in solution?

    Yes. A 0.5 M stock solution at pH 8.5 is stable for months at 2-8°C.

  5. What is the working concentration for Product E5134, EDTA disodium salt, for use as an anti-coagulant?

    The optimal concentration is 1.5 mg per mL of blood.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Clinical Chemistry, 45(9), 1536-1542 (1999)
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Nature protocols, 1(2), 870-873 (2007-04-05)
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Nature protocols, 1(2), 954-959 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that consists of numerous uncultured microbes. Therefore, nucleic-acid-based approaches have been introduced to study microbial diversity and activity, and these depend on the proper isolation of DNA, rRNA and mRNA....

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