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Monoclonal ANTI-FLAG® antibody produced in rabbit

clone SIG1-25, ascites fluid

Anti-ddddk, Anti-dykddddk

origen biológico


Nivel de calidad



forma del anticuerpo

ascites fluid

antibody product type

primary antibodies


SIG1-25, monoclonal


immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton X-100
indirect ELISA: suitable
western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein



secuencia del inmunógeno


enviado en

dry ice

temp. de almacenamiento


Related Categories

Descripción general

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG® peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in association with other tags. The small size of the FLAG tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function. The N-terminal FLAG® peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage catalyzed by Cu2+ ions of the C-terminal FLAG® peptide from a fusion protein has been reported. A sequence motif with five out of eight amino acid residues identical to the FLAG® peptide is found in both rat and mouse Mg2+ dependent protein b-phosphatase, as well as in the human and bovine enzyme. This rabbit ANTI-FLAG® antibody is produced from the hybridoma SIG1-25. The hybridoma is derived by the fusion of myeloma cells and splenocytes from rabbits immunized with the FLAG® peptide sequence conjugated to KLH.


This monoclonal antibody recognizes the FLAG sequence at the N-terminus


FLAG peptide sequence DYKDDDDK


Monoclonal ANTI-FLAG® antibody produced in rabbit has been used in:
  • immunofluorescence
  • western blot analysis
  • immunocytochemistry
  • indirect ELISA

Browse additional application references in our FLAG® Literature portal.


100, 200 μL

Forma física

Provided as ascites fluid containing 15 mM sodium azide as a preservative.

Información legal

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Código de clase de almacenamiento

12 - Non Combustible Liquids



Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

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Certificate of Origin

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