Merck

F2555

Sigma-Aldrich

Rabbit Monoclonal ANTI-FLAG® Clone SIGI-25

clone SIG1-25, ascites fluid

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Sinónimos:
Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SIG1-25, monoclonal

technique(s)

immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton X-100
indirect ELISA: suitable
western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein

isotype

IgG

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

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Este artículo
F3040SAB4301135A9469
clone

SIG1-25, monoclonal

clone

M1, monoclonal

clone

polyclonal

clone

M2, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

alkaline phosphatase conjugate

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

mouse

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

technique(s)

immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton X-100, western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein, indirect ELISA: suitable

technique(s)

western blot: 10 μg/mL

technique(s)

western blot: 1:1000-1:5000 (Cell Lysate)

technique(s)

indirect ELISA: 1:20,000

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General description

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG® peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in association with other tags. The small size of the FLAG tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function. The N-terminal FLAG® peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage catalyzed by Cu2+ ions of the C-terminal FLAG® peptide from a fusion protein has been reported. A sequence motif with five out of eight amino acid residues identical to the FLAG® peptide is found in both rat and mouse Mg2+ dependent protein b-phosphatase, as well as in the human and bovine enzyme. This rabbit ANTI-FLAG® antibody is produced from the hybridoma SIG1-25. The hybridoma is derived by the fusion of myeloma cells and splenocytes from rabbits immunized with the FLAG® peptide sequence conjugated to KLH.

Specificity

This monoclonal antibody recognizes the FLAG sequence at the N-terminus

Immunogen

FLAG peptide sequence DYKDDDDK

Application

Monoclonal ANTI-FLAG® antibody produced in rabbit has been used in:

  • immunofluorescence
  • western blot analysis
  • immunocytochemistry
  • indirect ELISA

Learn more product details in our FLAG® application portal.

Physical form

Provided as ascites fluid containing 15 mM sodium azide as a preservative.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Jennifer J Banerjee et al.
eLife, 6 (2017-07-01)
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Reduction of NANOG mediates the inhibitory effect of aspirin on tumor growth and stemness in colorectal cancer
Wang H, et al.
Cellular Physiology and Biochemistry, 44(3), 1051-1063 (2017)
Qungang Qi et al.
The Journal of biological chemistry, 287(37), 31482-31493 (2012-07-18)
Previous studies have demonstrated that Arabidopsis thaliana BBX32 (AtBBX32) represses light signaling in A. thaliana and that expression of AtBBX32 in soybean increases grain yield in multiple locations and multiyear field trials. The BBX32 protein is a member of the
Recombinant lentivirus with enhanced expression of caudal-related homeobox protein 2 inhibits human colorectal cancer cell proliferation in vitro
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PLoS ONE, 8(9), e75137-e75137 (2013)

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