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F3165

Sigma-Aldrich

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

Synonym(s):
Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

origen biológico

mouse

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin (Purified IgG1 subclass)

antibody product type

primary antibodies

clon

M2, monoclonal

formulario

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

purificado por

using Protein A

species reactivity

all

concentración

3.8-4.2 mg/mL

technique(s)

western blot: 10 μg/mL (Protein A)

isotipo

IgG1

secuencia del inmunógeno

DYKDDDDK

enviado en

dry ice

temp. de almacenamiento

−20°C

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Descripción general

Anti Flag M2 antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is produced in mouse and recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus. The antibody is also able to recognize FLAG at an internal site. M2, unlike M1 antibody is not Calcium dependent.
F1804 is an affinity purified, FLAG M2 antibody, increasing sensitivity in most applications.
Method of purification - Protein A

Inmunógeno

FLAG; peptide sequence DYKDDDDK

Aplicación

Monoclonal ANTI-FLAG® M2 antibody produced in mouse has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry
  • immunofluorescence
  • ELISA
  • EIA
  • chromatin immunoprecipitation
  • electron microscopy
  • flow cytometry
  • supershift assays

Browse additional application references in our FLAG® Literature portal.

Envase

5 mg in poly bottle

Nota de preparación

Dilute the antibody solution from 0.5-10 ug/mL in Tris Buffered Saline, pH 8.0, with 3% nonfat milk

Almacenamiento y estabilidad

Store the undiluted antibody at –20 °C in working aliquots. Repeated freezing and thawing is not recommended.
Note: Overtime, small amounts of purified antibodies can precipitate from solution due to intermolecular hydrophobic interactions. If a precipitate is observed in this product, briefly centrifuge the vial to pellet the precipitate. Withdraw the desired volume of antibody solution from the clear supernatant for use. This should not alter the performance of the purified antibody in Western blot or immunoprecipitation applications.

Información legal

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

nwg

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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