F4049

Monoclonal ANTI-FLAG^® M2-FITC antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Monoclonal ANTI-FLAG^® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk
• NACRES: NA.32

Properties

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: FITC conjugate
• forma del anticuerpo: purified immunoglobulin
• antibody product type: primary antibodies
• clon: M2, monoclonal
• formulario: buffered aqueous solution
• species reactivity: all
• concentración: ~1 mg/mL
• technique(s): direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
• isotipo: IgG1
• secuencia del inmunógeno: DYKDDDDK
• enviado en: dry ice
• temp. de almacenamiento: −20°C

Description

Descripción general

Anti Flag M2-FITC antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is produced in mouse and covalently conjugated to flourescein isothiocyanate (FITC). The antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus.Binding is not Calcium dependent. F4049 is an Affinity purified antibody, increasing sensitivity in most applications.

Aplicación

Antibody is suitable for immunofluorescence, immunocytochemistry, immunohistochemistry, immunoblotting, flow cytometry and electron microscopy. Ideal for use when detection of Flag fusion proteins is required in a murine host, where secondary antibodies could cause cross reactivity.

Browse additional application references in our FLAG^® Literature portal.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Envase

0.2, 1, 1 mg in poly bottle

Forma física

Solution in 10 mM phosphate buffered saline, plus 1% BSA and 0.1% sodium azide, pH 7.4

Información legal

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable