Merck
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F4135

Sigma-Aldrich

Fetal Bovine Serum

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

Synonym(s):
HI FBS, HI FCS, HI sera, HI serum, FBS, FCS, sera, serum
Número MDL:
eCl@ss:
32160409
NACRES:
NA.71

origen biológico

bovine fetus

Nivel de calidad

500

esterilidad

sterile-filtered

línea de producto

FBS Classic

composición

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

origen

USA origin

technique(s)

cell culture | hybridoma: suitable
cell culture | insect: suitable
cell culture | mammalian: suitable

impurezas

≤10 EU/mL endotoxin

enviado en

dry ice

temp. de almacenamiento

−20°C

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Descripción general

Fetal Bovine Serum (FBS) remains a popular media supplement as it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and aids in proliferation. It also protects cells from oxidative damage and apoptosis. FBS blocks reactive oxygen species generation, which otherwise is not eliminated in serum-free media (SFM) systems.

Aplicación

FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used:
  • as a component of Dulbecco′s Modified Eagles Media (DME, DMEM), Eagle′s Minimum Essential Medium (EMEM), Roswell Park Memorial Institute medium (RPMI-1640), α-modified eagle medium (αMEM) (MEM) and Leibovitz L-15 medium
  • as a component of blocking medium for treating brainstem slices
  • to improve attachment of dermal explants

Nota de preparación

Prepared by heating at 56°C for 30 minutes.
This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Nota de análisis

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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