F9037

Formamide

≥99.5% (GC), BioReagent, for molecular biology

• Synonym(s): Amide C₁, Formic amide
• Linear Formula: HCONH₂
• CAS Number: 75-12-7
• Molecular Weight: 45.04
• Beilstein: 505995
• Número de EC: 200-842-0
• Número MDL: MFCD00007941
• ID de la sustancia en PubChem: 24894985

Properties

• grado: for molecular biology
• Nivel de calidad: 200
• densidad de vapor: 1.55 (vs air)
• presión de vapor: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• Línea del producto: BioReagent
• ensayo: ≥99.5% (GC)
• formulario: liquid
• temp. de autoignición: 932 °F
• technique(s): electrophoresis: suitable; immunohistochemistry: suitable
• índice de refracción: n20/D 1.447 (lit.)
• pH: 4-10 (20 °C, 200 g/L)
• bp: 210 °C (lit.)
• mp: 2-3 °C (lit.)
• densidad: 1.134 g/mL at 25 °C (lit.)
• idoneidad: suitable for nucleic acid hybridization
• temp. de almacenamiento: 2-8°C
• SMILES string: NC=O
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• InChI key: ZHNUHDYFZUAESO-UHFFFAOYSA-N

Description

Descripción general

Formamide is a clear liquid amide derived from formic acid. Formamide allows for the denaturation and renaturation of nucleic acids at room temperature.

Aplicación

Formamide is suitable for use in hybridization buffers made using standard recipes. Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Other applications include:

• Preparation of brain sections of mice for BrdU immunohistochemistry
• FISH analysis of HeLa cells.
• Preparation of the polyacrylamide gel for analysis of PCR products using denaturing gradient gel electrophoresis(The Merck Index, 12th ed).

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Envase

100 mL

Características y beneficios

• Deionized for molecular biology applications
• Packaged under argon gas in amber bottles to protect from oxidation
• Used for DNA renaturation or DNA-RNA hybridization
• Reduces thermal stability of double stranded nucleic acids

Idoneidad

• nucleic acid hybridization
• cryoprotectant and gel-stabilizer
• destabilize nucleic acid duplexes
• elimination of secondary structure of nucleic acids
• preparation of hybridization buffers for RNA and DNA application

Otras notas

The product has been deionized for molecular biology use and packaged under argon gas in amber glass bottles to protect it from oxidation.

Safety Information

• pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H351 - H360D - H373
• Consejos de prudencia: P202 - P260 - P280 - P308 + P313 - P405 - P501
• Clasificaciones de peligro: Carc. 2 - Repr. 1B - STOT RE 2 Oral
• Órganos de actuación: Blood
• Código de clase de almacenamiento: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Punto de inflamabilidad F: 305.6 °F
• Punto de inflamabilidad C: 152 °C
• Equipo de protección personal: Eyeshields, Gloves, type ABEK (EN14387) respirator filter