F9291

Monoclonal ANTI-FLAG^® BioM2 antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

• Synonym(s): Monoclonal ANTI-FLAG^® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk

Properties

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: biotin conjugate
• forma del anticuerpo: purified immunoglobulin
• antibody product type: primary antibodies
• clon: M2, monoclonal
• formulario: buffered aqueous glycerol solution
• species reactivity: all
• concentración: ~1 mg/mL
• technique(s): dot blot: suitable (chemiluminescent detection)
• isotipo: IgG1
• secuencia del inmunógeno: DYKDDDDK
• enviado en: dry ice
• temp. de almacenamiento: −20°C

Description

Descripción general

The monoclonal Anti-FLAG BioM2 mouse antibody is covalently attached to biotin by hydrazide linkage. The antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus or C-terminus of FLAG fusion porteins.

Aplicación

Biotin-labeled antibody is used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host.
Antibody is suitable for immunofluorescence, western blotting, microscopy applications and for the formation of avidin-biotin complexes.

Browse additional application references in our FLAG^® Literature portal.

Forma física

Solution in 50% glycerol, 10 mM sodium phosphate, pH 7.25, containing 150 mM NaCl and 0.02% sodium azide

Nota de preparación

Dilute antibody in TBS (.05M Tris, pH7.4, with .15M NaCl) to a final concentration of 1-10μg/mL.

Información legal

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 2
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable