Merck
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F9291

Sigma-Aldrich

Monoclonal ANTI-FLAG® BioM2 antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk

origen biológico

mouse

Nivel de calidad

200

conjugado

biotin conjugate

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

M2, monoclonal

formulario

buffered aqueous glycerol solution

species reactivity

all

concentración

~1 mg/mL

technique(s)

dot blot: suitable (chemiluminescent detection)

isotipo

IgG1

secuencia del inmunógeno

DYKDDDDK

enviado en

dry ice

temp. de almacenamiento

−20°C

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Descripción general

The monoclonal Anti-FLAG BioM2 mouse antibody is covalently attached to biotin by hydrazide linkage. The antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus or C-terminus of FLAG fusion porteins.

Aplicación

Biotin-labeled antibody is used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host.
Antibody is suitable for immunofluorescence, western blotting, microscopy applications and for the formation of avidin-biotin complexes.

Browse additional application references in our FLAG® Literature portal.

Forma física

Solution in 50% glycerol, 10 mM sodium phosphate, pH 7.25, containing 150 mM NaCl and 0.02% sodium azide

Nota de preparación

Dilute antibody in TBS (.05M Tris, pH7.4, with .15M NaCl) to a final concentration of 1-10μg/mL.

Información legal

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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