G0751

β-Glucuronidase from Helix pomatia

Type H-1, partially purified powder, ≥300,000 units/g solid

• Synonym(s): β-D-Glucuronide glucuronosohydrolase
• CAS Number: 9001-45-0
• Enzyme Commission number: 3.2.1.31 (BRENDA, IUBMB)
• Número de EC: 232-606-8
• Número MDL: MFCD00130629
• NACRES: NA.54

Properties

• tpo: Type H-1
• Nivel de calidad: 200
• formulario: partially purified powder
• specific activity: ≥300,000 units/g solid
• secondary activity: ≥10,000 units/g solid sulfatase
• temp. de almacenamiento: −20°C

Description

Descripción general

β-Glucuronidase Type H-1 from Helix pomatia is partially purified powder containing enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Envase

100000, 500000, 1000000, 2000000 units in poly bottle

Aplicación

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Acciones bioquímicas o fisiológicas

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Calidad

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Definición de unidad

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Safety Information

• pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H317 - H334
• Consejos de prudencia: P261 - P280 - P342 + P311
• Clasificaciones de peligro: Resp. Sens. 1 - Skin Sens. 1
• Código de clase de almacenamiento: 13 - Non Combustible Solids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)