G0799

β-Glucuronidase from Escherichia coli

>20,000,000 units/g protein, recombinant, expressed in E. coli, aqueous glycerol solution

• Synonym(s): β-D-Glucuronide glucuronosohydrolase
• CAS Number: 9001-45-0
• Enzyme Commission number: 3.2.1.31 (BRENDA, IUBMB)
• Número de EC: 232-606-8
• Número MDL: MFCD00130629
• NACRES: NA.54

Properties

• recombinante: expressed in E. coli
• Nivel de calidad: 200
• formulario: aqueous glycerol solution
• specific activity: >20,000,000 units/g protein
• mol peso: 74 kDa
• concentración: >1 mg/mL
• enviado en: wet ice
• temp. de almacenamiento: −20°C

Description

Descripción general

β-Glucuronidase is a tetramer belonging to the glycosidase family with a molecular weight of 29 kDa. It is composed of 74 kDa identical monomers. The pI and optimal pH of the enzyme is 4.8 and 6-7 respectively.

Aplicación

β-Glucuronidase was used in the study of Cer-β-glucuronide synthesis and evaluation of β-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.

New Technical Article Comparing Performance of Different Enzymes
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about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Effective in the hydrolysis of steroid glucuronides.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Envase

25000, 100000 units in poly bottle

Definición de unidad

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

Forma física

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Highly purified solution in 50% glycerol

Safety Information

• pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H317 - H334
• Consejos de prudencia: P261 - P280 - P342 + P311
• Clasificaciones de peligro: Resp. Sens. 1 - Skin Sens. 1
• Código de clase de almacenamiento: 13 - Non Combustible Solids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable