Merck
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G1512

Sigma-Aldrich

β-Glucuronidase from Helix pomatia

Type H-5, lyophilized powder, ≥400,000 units/g solid

Synonym(s):
β-D-Glucuronide glucuronosohydrolase
CAS Number:
Enzyme Commission number:
Número de EC:
Número MDL:
NACRES:
NA.54

tpo

Type H-5

Nivel de calidad

formulario

lyophilized powder

specific activity

≥400,000 units/g solid

secondary activity

≤40,000 units/g solid sulfatase

temp. de almacenamiento

−20°C

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Descripción general

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Aplicación

β-Glucuronidase from Helix pomatia has been used:
  • in the enzymatic treatment of urine samples for liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis
  • in the quercetin extraction procedure from pig or rat tissues for high performance liquid chromatography (HPLC) analysis
  • in the enzymatic deconjugation step in urine and plasma sample preparation for gas chromatography-mass spectrometry analysis (GC-MS)

β-glucuronidase was used to hydrolyze conjugated glucuronide and sulfate metabolites.
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Envase

50000, 100000, 500000 units in poly bottle

Acciones bioquímicas o fisiológicas

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Calidad

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Ligadura / enlace

A further purification of G0876 by gel permeation chromatography.

Definición de unidad

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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